PXD016878 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | IP C5ORF30 macrophage immunometabolism regulator gene |
Description | Expression of the macrophage immunometabolism regulator gene (MACIR) is associated with severity of autoimmune disease pathology and the regulation of macrophage biology through unknown mechanisms. The 206 amino acid protein lacks homology to any characterized protein sequence and is a disordered protein according to structure prediction algorithms. Here we identify specific interactions of MACIR using a fragment complementation-based affinity pull down of cellular proteins prepared with a membrane solubilization buffer. Quantitative mass spectrometry showed enrichment of nuclear and mitochondrial proteins and of 63 significant interacting proteins, binding to the nuclear transport receptor TNPO1 and trafficking proteins UNC119 homolog A and B were validated by immunoprecipitation. Analysis of mutations in two candidate recognition motifs in the MACIR amino acid sequence confirmed TNPO1 binds via a PY-NLS motif (aa98-117). Characterizing nuclear MACIR activity in macrophage and fibroblasts is a priority with respect to developing strategies for treatment of autoimmune disease. |
HostingRepository | PRIDE |
AnnounceDate | 2020-09-28 |
AnnouncementXML | Submission_2020-09-28_05:37:23.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | David Matallanas |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-20 05:09:19 | ID requested | |
⏵ 1 | 2020-09-28 05:37:24 | announced | |
Publication List
McGauran G, Dorris E, Borza R, Morgan N, Shields DC, Matallanas D, Wilson AG, O'Connell DJ, Resolving the Interactome of the Human Macrophage Immunometabolism Regulator (MACIR) with Enhanced Membrane Protein Preparation and Affinity Proteomics. Proteomics, 20(19-20):e2000062(2020) [pubmed] |
Keyword List
submitter keyword: IP C5ORF30 MACIR Human Rheumatoid Arthritis |
Contact List
David Gomez |
contact affiliation | Systems Biology Ireland, University College Dublin |
contact email | david.gomez@ucd.ie |
lab head | |
David Matallanas |
contact affiliation | Systems Biology Ireland, University College Dublin |
contact email | david.gomez@ucd.ie |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD016878 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016878
- Label: PRIDE project
- Name: IP C5ORF30 macrophage immunometabolism regulator gene