PXD016680-1

PXD016680 is an original dataset announced via ProteomeXchange.

Title	An interaction network of RNA-binding proteins involved in Drosophila oogenesis
Description	During Drosophila oogenesis, the localization and translational regulation of maternal transcripts relies on RNA-binding proteins (RBPs). Many of these RBPs localize several mRNAs and may have additional direct interaction partners to regulate their functions. Using immunoprecipitation from whole Drosophila ovaries coupled to mass spectrometry, we examined protein-protein associations of 6 GFP-tagged RBPs expressed at physiological levels. Analysis of the interaction network and further validation in human cells allowed us to identify 26 previously unknown associations, besides recovering several well characterized interactions.
HostingRepository	PRIDE
AnnounceDate	2020-06-22
AnnouncementXML	Submission_2020-06-21_22:27:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicolas Nalpas
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-10 07:12:44	ID requested
⏵ 1	2020-06-21 22:28:00	announced
2	2024-10-22 05:07:51	announced	2024-10-22: Updated project metadata.

Bansal P, Madlung J, Schaaf K, Macek B, Bono F, Oogenesis. Mol Cell Proteomics, 19(9):1485-1502(2020) [pubmed]

curator keyword: Biological

submitter keyword: Drosophila melanogaster, transgenic fly lines, oogenesis, RNA-binding proteins, immunoprecipitation, LC/MS, in vitro validation

Fulvia Bono
contact affiliation	Living Systems Institute University of Exeter Exeter UK
contact email	F.Bono@exeter.ac.uk
lab head
Nicolas Nalpas
contact affiliation	Tuebingen University
contact email	nalpas.nicolas@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD016680

PRIDE project URI

• PRIDE
  1. PXD016680
     1. Label: PRIDE project
     2. Name: An interaction network of RNA-binding proteins involved in Drosophila oogenesis