PXD016158-2

PXD016158 is an original dataset announced via ProteomeXchange.

Title	Quantification of exercise-regulated ubiquitin-signalling
Description	The maintenance of muscle function is extremely important for whole body health and exercise is essential to this process. The ubiquitin-proteasome system (UPS) is required for muscle adaptation following exercise but little is known about acute post-translational regulation and proteome remodelling during exercise. Here, we used quantitative proteomics to study ubiquitin-signalling dynamics in human skeletal muscle biopsies from healthy males before, during and after a single bout of high-intensity exercise. Exercise resulted in a marked depletion of protein ubiquitylation consistent with proteasome activation. This was also associated with acute post-translational modification of protein abundance via a network of proteases.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:00:51.830.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Parker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-05 08:15:40	ID requested
1	2020-03-17 03:57:45	announced
⏵ 2	2024-10-22 05:00:52	announced	2024-10-22: Updated project metadata.

10.1096/fj.202000075r;

Parker BL, Kiens B, Wojtaszewski JFP, Richter EA, James DE, Quantification of exercise-regulated ubiquitin signaling in human skeletal muscle identifies protein modification cross talk via NEDDylation. FASEB J, 34(4):5906-5916(2020) [pubmed]

submitter keyword: proteomics,exercise, ubiquitin

Benjamin Parker
contact affiliation	The University of Melbourne and University of Sydney
contact email	ben.parker@unimelb.edu.au
lab head
Benjamin Parker
contact affiliation	The University of Melbourne
contact email	ben.parker@unimelb.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016158
     1. Label: PRIDE project
     2. Name: Quantification of exercise-regulated ubiquitin-signalling