PXD016158-1

PXD016158 is an original dataset announced via ProteomeXchange.

Title	Quantification of exercise-regulated ubiquitin-signalling
Description	The maintenance of muscle function is extremely important for whole body health and exercise is essential to this process. The ubiquitin-proteasome system (UPS) is required for muscle adaptation following exercise but little is known about acute post-translational regulation and proteome remodelling during exercise. Here, we used quantitative proteomics to study ubiquitin-signalling dynamics in human skeletal muscle biopsies from healthy males before, during and after a single bout of high-intensity exercise. Exercise resulted in a marked depletion of protein ubiquitylation consistent with proteasome activation. This was also associated with acute post-translational modification of protein abundance via a network of proteases.
HostingRepository	PRIDE
AnnounceDate	2020-03-17
AnnouncementXML	Submission_2020-03-17_03:57:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Parker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-05 08:15:40	ID requested
⏵ 1	2020-03-17 03:57:45	announced
2	2024-10-22 05:00:52	announced	2024-10-22: Updated project metadata.

Parker BL, Kiens B, Wojtaszewski JFP, Richter EA, James DE, Quantification of exercise-regulated ubiquitin signaling in human skeletal muscle identifies protein modification cross talk via NEDDylation. FASEB J, 34(4):5906-5916(2020) [pubmed]

submitter keyword: exercise, ubiquitin, proteomics

Benjamin Parker
contact affiliation	The University of Melbourne and University of Sydney
contact email	ben.parker@unimelb.edu.au
lab head
Benjamin Parker
contact affiliation	The University of Melbourne
contact email	ben.parker@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD016158

PRIDE project URI

• PRIDE
  1. PXD016158
     1. Label: PRIDE project
     2. Name: Quantification of exercise-regulated ubiquitin-signalling