PXD016073-2

PXD016073 is an original dataset announced via ProteomeXchange.

Title	Identification of novel serum biomarkers for malignant mesothelioma using iTRAQ combined with 2D-LC-MS/MS
Description	In this project, differential serum proteins (DEPs) were first screened in MM patients and healthy controls using iTRAQ labeling coupled with 2D LC-MS/MS method. As a result, 475 proteins were identified, of which, 327 proteins contain at least two unique peptides, and 145 DEPs were found between MM patients and healthy controls. Besides, all the identified proteins were subjected to bioinformatic analysis by using the Gene Ontology(GO) database，the Kyoto Encyclopedia of Genes Genomes（KEGG）database and the Cluster of Orthologous Groups of proteins(COG) database to find the relative processes that these proteins were involved in. Furthermore, function enrichment analysis (GO enrichment and KEGG enrichment) of these DEPs showed enrichment in several biological pathways which can characterize the most significant functional roles and related pathways. Candidate protein biomarkers were selected for subsequent validation in large cohorts of patients with asbestos related diseases by ELISA assay. Our findings indicated that Serum FLNA, FBLN1 and TSP-1 might be promising candidates for diagnosis of MM and screening of at-risk individuals.
HostingRepository	iProX
AnnounceDate	2019-10-30
AnnouncementXML	Submission_2023-08-27_21:04:36.404.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hailing Xia
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-30 01:21:44	ID requested
1	2019-10-30 01:23:47	announced
⏵ 2	2023-08-27 21:04:37	announced	2023-08-28: Update publication information.

Xia H, Feng L, Lin L, Jiang Z, Chen J, Shi W, Ying S, Yu M, Ju L, Zhu L, Shi L, Zhang X, Lou J, Exploration of identifying novel serum biomarkers for malignant mesothelioma using iTRAQ combined with 2D-LC-MS/MS. Environ Res, 193():110467(2021) [pubmed]

submitter keyword: Serum, iTRAQ, biomarker, malignant mesothelioma

Jianlin Lou
contact affiliation	Zhejiang Academy of Medical Sciences
contact email	jianlin@163.com
lab head
Hailing Xia
contact affiliation	Zhejiang Academy of Medical Sciences
contact email	zhangxia0419@163.com
dataset submitter

iProX dataset URI