PXD015996-2

PXD015996 is an original dataset announced via ProteomeXchange.

Title	Aberrant astrocyte protein secretion contributes to altered neuronal development in diverse disorders
Description	Astrocytes negatively impact neuronal development in a range of neurodevelopmental disorders (NDs), however how they do this, and if mechanisms are shared across multiple disorders, is not known. We developed an in vitro system to ask how astrocyte protein secretion and gene expression change in 3 genetic NDs. This identified disorder specific changes, as well as core proteins that are increased in release in all 3 NDs. ND astrocytes increase release of Igfbp2, and blocking Igfbp2 partially rescues the neurite outgrowth inhibitory effects of Rett Syndrome astrocytes. We identified that ND astrocytes upregulate release of Bmp6, which acts on astrocytes themselves and is upstream of astrocyte secretion changes in NDs. Blocking Bmp signaling in Fragile X Syndrome astrocytes reverses the inhibitory effects of FXS astrocytes on neuronal development. We provide a resource of astrocyte secreted proteins in health and NDs, as well as novel targets for intervention in diverse NDs.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:38:15.677.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jolene Diedrich
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-24 02:00:11	ID requested
1	2022-07-11 14:35:31	announced
⏵ 2	2024-10-22 05:38:16	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: neurodevelopment, synapse, secreted proteins, axon, IGF, BMP,Astrocyte, dendrite

Nicola Allen
contact affiliation	Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA
contact email	nallen@salk.edu
lab head
Jolene Diedrich
contact affiliation	Salk
contact email	jdiedrich@salk.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD015996

PRIDE project URI

• PRIDE
  1. PXD015996
     1. Label: PRIDE project
     2. Name: Aberrant astrocyte protein secretion contributes to altered neuronal development in diverse disorders