PXD015996 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Aberrant astrocyte protein secretion contributes to altered neuronal development in diverse disorders |
Description | Astrocytes negatively impact neuronal development in a range of neurodevelopmental disorders (NDs), however how they do this, and if mechanisms are shared across multiple disorders, is not known. We developed an in vitro system to ask how astrocyte protein secretion and gene expression change in 3 genetic NDs. This identified disorder specific changes, as well as core proteins that are increased in release in all 3 NDs. ND astrocytes increase release of Igfbp2, and blocking Igfbp2 partially rescues the neurite outgrowth inhibitory effects of Rett Syndrome astrocytes. We identified that ND astrocytes upregulate release of Bmp6, which acts on astrocytes themselves and is upstream of astrocyte secretion changes in NDs. Blocking Bmp signaling in Fragile X Syndrome astrocytes reverses the inhibitory effects of FXS astrocytes on neuronal development. We provide a resource of astrocyte secreted proteins in health and NDs, as well as novel targets for intervention in diverse NDs. |
HostingRepository | PRIDE |
AnnounceDate | 2022-07-11 |
AnnouncementXML | Submission_2022-07-11_14:35:31.434.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jolene Diedrich |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-24 02:00:11 | ID requested | |
⏵ 1 | 2022-07-11 14:35:31 | announced | |
2 | 2024-10-22 05:38:16 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Astrocyte, neurodevelopment, synapse, axon, dendrite, IGF, BMP, secreted proteins |
Contact List
Nicola Allen |
contact affiliation | Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA |
contact email | nallen@salk.edu |
lab head | |
Jolene Diedrich |
contact affiliation | Salk |
contact email | jdiedrich@salk.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD015996 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015996
- Label: PRIDE project
- Name: Aberrant astrocyte protein secretion contributes to altered neuronal development in diverse disorders