PXD015674-1

PXD015674 is an original dataset announced via ProteomeXchange.

Title	Rapid and quantitative precipitation of a yeast proteome
Description	Protein precipitation has applications as a front-end preparation strategy for proteome analysis, as well as depleting proteins in serum for small molecule analysis or for commercial preparation of bulk protein. The highly variable conditions previously implemented to precipitate proteins are reflected by inconsistent and low recovery, as well as incomplete proteome coverage, diminishing the utility of precipitation for sample preparation. We herein investigate and optimize the conditions affecting protein recovery, using acetone at a defined ionic strength. We show rapid (2 min) precipitation at room temperature provides consistently high protein recovery (98 +/- 1%), with MS protein identifications equivalent to overnight, -20 °C incubations. Our robust strategy to isolate proteins maximizes recovery with minimal structural bias, exploiting the analytical advantages of precipitation over alternative techniques.
HostingRepository	PRIDE
AnnounceDate	2020-03-23
AnnouncementXML	Submission_2020-03-23_00:05:25.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015674
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jessica Nickerson
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	Phospho; Met-loss; Met-loss+Acetyl; Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-02 01:47:09	ID requested
⏵ 1	2020-03-23 00:05:27	announced

Nickerson JL, Doucette AA, Rapid and Quantitative Protein Precipitation for Proteome Analysis by Mass Spectrometry. J Proteome Res, 19(5):2035-2042(2020) [pubmed]

submitter keyword: acetone, salt, temperature, throughput, bottom-up

Alan Austin Doucette
contact affiliation	Department of Chemistry, Dalhousie University, Halifax, Canada
contact email	alan.doucette@dal.ca
lab head
Jessica Nickerson
contact affiliation	Dalhousie University
contact email	js860572@dal.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015674
     1. Label: PRIDE project
     2. Name: Rapid and quantitative precipitation of a yeast proteome