PXD014758 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cytosolic and plasma membrane proteome analysis of rice leaves in response to MSP1 treatment |
Description | MSP1 is a Magnaporthe oryzae secreted protein that elicits defense responses in rice. However, the molecular mechanism of MSP1 action is largely elusive. Here, we employed a TMT-based quantitative proteomic analysis of cytoplasmic as well as plasma membrane proteins to decipher the MSP1 induced signalling in rice. This approach led to the identification of 6691 proteins of which 3049 were identified in the plasma membrane (PM) while 3642 were identified in the cytoplasmic fraction. A parallel phosphoproteome analysis led to the identification of 1906 phosphopeptides and integration of proteome and phosphoproteome data showed activation of proteins related to the proteolysis, jasmonic acid biosynthesis, redox metabolism and MAP kinase signaling pathways in response to MSP1 treatment. Further, MSP1 induced phosphorylation of some of the key proteins including RBOHB, MEKK1, MPK3/6, CDPK and CaM suggest activation of PAMP-triggered immunity (PTI) in response to MSP1 treatment. In essence, our results further support the functioning of MSP1 as a PAMP and provide an overview of the MSP1 induced signaling in rice leaves. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:57:54.881.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sun Tae Kim |
SpeciesList | scientific name: Oryza sativa (Rice); NCBI TaxID: 4530; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-25 02:57:53 | ID requested | |
1 | 2019-08-30 01:29:08 | announced | |
⏵ 2 | 2024-10-22 04:57:55 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.3390/ijms20174135; |
Gupta R, Min CW, Kim YJ, Kim ST, Identification of Msp1-Induced Signaling Components in Rice Leaves by Integrated Proteomic and Phosphoproteomic Analysis. Int J Mol Sci, 20(17):(2019) [pubmed] |
Keyword List
submitter keyword: MSP1, rice, TMT,plasma membrane, plant-pathogen interaction, PAMP |
Contact List
Sun Tae Kim |
contact affiliation | Pusan National University, South Korea |
contact email | stkim5505@gmail.com |
lab head | |
Sun Tae Kim |
contact affiliation | Pusan National University |
contact email | stkim5505@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014758
- Label: PRIDE project
- Name: Cytosolic and plasma membrane proteome analysis of rice leaves in response to MSP1 treatment