PXD014758-1

PXD014758 is an original dataset announced via ProteomeXchange.

Title	Cytosolic and plasma membrane proteome analysis of rice leaves in response to MSP1 treatment
Description	MSP1 is a Magnaporthe oryzae secreted protein that elicits defense responses in rice. However, the molecular mechanism of MSP1 action is largely elusive. Here, we employed a TMT-based quantitative proteomic analysis of cytoplasmic as well as plasma membrane proteins to decipher the MSP1 induced signalling in rice. This approach led to the identification of 6691 proteins of which 3049 were identified in the plasma membrane (PM) while 3642 were identified in the cytoplasmic fraction. A parallel phosphoproteome analysis led to the identification of 1906 phosphopeptides and integration of proteome and phosphoproteome data showed activation of proteins related to the proteolysis, jasmonic acid biosynthesis, redox metabolism and MAP kinase signaling pathways in response to MSP1 treatment. Further, MSP1 induced phosphorylation of some of the key proteins including RBOHB, MEKK1, MPK3/6, CDPK and CaM suggest activation of PAMP-triggered immunity (PTI) in response to MSP1 treatment. In essence, our results further support the functioning of MSP1 as a PAMP and provide an overview of the MSP1 induced signaling in rice leaves.
HostingRepository	PRIDE
AnnounceDate	2019-08-30
AnnouncementXML	Submission_2019-08-30_01:29:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sun Tae Kim
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-25 02:57:53	ID requested
⏵ 1	2019-08-30 01:29:08	announced
2	2024-10-22 04:57:55	announced	2024-10-22: Updated project metadata.

Gupta R, Min CW, Kim YJ, Kim ST, Identification of Msp1-Induced Signaling Components in Rice Leaves by Integrated Proteomic and Phosphoproteomic Analysis. Int J Mol Sci, 20(17):(2019) [pubmed]

submitter keyword: plasma membrane, TMT, MSP1, rice, plant-pathogen interaction, PAMP

Sun Tae Kim
contact affiliation	Pusan National University, South Korea
contact email	stkim5505@gmail.com
lab head
Sun Tae Kim
contact affiliation	Pusan National University
contact email	stkim5505@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014758
     1. Label: PRIDE project
     2. Name: Cytosolic and plasma membrane proteome analysis of rice leaves in response to MSP1 treatment