PXD014195-1

PXD014195 is an original dataset announced via ProteomeXchange.

Title	Fibro-adipogenic progenitors of dystrophic mice are insensitive to NOTCH regulation of adipogenesis
Description	Fibro adipogenic progenitors (FAPs) promote satellite cell differentiation in adult skeletal muscle regeneration. However, in pathological conditions, FAPs are responsible for fibrosis and fatty infiltrations. Here we show that the NOTCH pathway negatively modulates FAP differentiation both in vitro and in vivo. However, FAPs isolated from young dystrophin- deficient mdx mice are insensitive to this control mechanism. An unbiased mass spectrometry-based proteomic analysis of FAPs from muscles of wild type and mdx mice, suggest that the synergistic cooperation between NOTCH and inflammatory signals controls FAP differentiation. Remarkably, we demonstrated that factors released by hematopoietic cells restore the sensitivity to NOTCH adipogenic inhibition in mdx FAPs. These results offer a basis for rationalizing pathological ectopic fat infiltrations in skeletal muscle and may suggest new therapeutic strategies to mitigate the detrimental effects of fat depositions in muscles of dystrophic patients.
HostingRepository	PRIDE
AnnounceDate	2019-06-24
AnnouncementXML	Submission_2019-06-24_06:39:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesca Sacco
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-10 01:40:27	ID requested
⏵ 1	2019-06-24 06:39:09	announced
2	2019-07-15 06:44:43	announced	Updated publication reference for PubMed record(s): 31239312.

Dataset with its publication pending

submitter keyword: adipogenesis, fibro adipogenic progenitors, inflammation, mass cytometry, skeletal muscle regeneration, muscular dystrophy, myopathies, NOTCH, proteome

Gianni Cesareni
contact affiliation	University of Rome Tor Vergata
contact email	cesareni@uniroma2.it
lab head
Francesca Sacco
contact affiliation	University of Rome Tor Vergata
contact email	fsacco@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD014195

PRIDE project URI

• PRIDE
  1. PXD014195
     1. Label: PRIDE project
     2. Name: Fibro-adipogenic progenitors of dystrophic mice are insensitive to NOTCH regulation of adipogenesis