⮝ Full datasets listing

PXD014125-2

PXD014125 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLabel-free quantitative proteomics in Candida yeast species: technical and biological replicates to asses data reproducibility
DescriptionObjective Label-free quantitative proteomics has emerged as a powerful strategy to obtain high quality quantitative measures of the proteome with only a very small quantity of total protein extract. Because our research projects were requiring the application of bottom-up shotgun Mass Spectrometry proteomics in the pathogenic yeasts Candida glabrata and Candida albicans, we performed preliminary experiments to i) obtained a precise list of all the proteins for which measures of abundance could be obtain and ii) assess the reproducibility of the results arising respectively from biological and technical replicates. Data description C. glabrata and C. albicans cells were grown in minimal liquid medium. Three independent time-courses were performed simultaneously, in each species, and an alkaline pH stress was induced for two of them. Cells were collected 10 and 60 minutes after stress induction and proteins were extracted. They were analysed and quantified two time by mass spectrometry. Our final dataset thus comprises label-free quantitative proteomics results for 24 samples (two species, three time-courses, two time points and two runs of mass spectrometry). Statistical procedures were applied to identify proteins with differential abundances between stressed and unstressed situations. Considering that C. glabrata and C. albicans are human pathogens which face important pH fluctuations during a human host infection, this dataset has a potential value to other researchers in the field.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:56:02.395.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThibaut LEGER
SpeciesList scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; scientific name: Candida glabrata (Yeast) (Torulopsis glabrata); NCBI TaxID: 5478;
ModificationListphosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-04 08:00:15ID requested
12019-07-08 00:38:21announced
22024-10-22 04:56:02announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mass spectrometry
label-free quantitative proteomics
Candida glabrata
Candida albicans
alkaline pH
Contact List
Pr. Gaëlle Lelandais
contact affiliationUniversité Paris-Sud Institut de Biologie Intégrative de la Cellule, campus Orsay
contact emailgaelle.lelandais@u-psud.fr
lab head
Thibaut LEGER
contact affiliationEHESP/LERES
contact emailthibaut.leger@ehesp.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD014125
PRIDE project URI
Repository Record List
[ + ]