PXD014125-1

PXD014125 is an original dataset announced via ProteomeXchange.

Title	Label-free quantitative proteomics in Candida yeast species: technical and biological replicates to asses data reproducibility
Description	Objective Label-free quantitative proteomics has emerged as a powerful strategy to obtain high quality quantitative measures of the proteome with only a very small quantity of total protein extract. Because our research projects were requiring the application of bottom-up shotgun Mass Spectrometry proteomics in the pathogenic yeasts Candida glabrata and Candida albicans, we performed preliminary experiments to i) obtained a precise list of all the proteins for which measures of abundance could be obtain and ii) assess the reproducibility of the results arising respectively from biological and technical replicates. Data description C. glabrata and C. albicans cells were grown in minimal liquid medium. Three independent time-courses were performed simultaneously, in each species, and an alkaline pH stress was induced for two of them. Cells were collected 10 and 60 minutes after stress induction and proteins were extracted. They were analysed and quantified two time by mass spectrometry. Our final dataset thus comprises label-free quantitative proteomics results for 24 samples (two species, three time-courses, two time points and two runs of mass spectrometry). Statistical procedures were applied to identify proteins with differential abundances between stressed and unstressed situations. Considering that C. glabrata and C. albicans are human pathogens which face important pH fluctuations during a human host infection, this dataset has a potential value to other researchers in the field.
HostingRepository	PRIDE
AnnounceDate	2019-07-08
AnnouncementXML	Submission_2019-07-08_00:38:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut LEGER
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; scientific name: [Candida] glabrata; NCBI TaxID: 5478;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-04 08:00:15	ID requested
⏵ 1	2019-07-08 00:38:21	announced
2	2024-10-22 04:56:02	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Mass spectrometry

label-free quantitative proteomics

Candida glabrata

Candida albicans

alkaline pH

Pr. Gaëlle Lelandais
contact affiliation	Université Paris-Sud Institut de Biologie Intégrative de la Cellule, campus Orsay
contact email	gaelle.lelandais@u-psud.fr
lab head
Thibaut LEGER
contact affiliation	Institut Jacques Monod
contact email	thibaut.leger@ijm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD014125

PRIDE project URI

• PRIDE
  1. PXD014125
     1. Label: PRIDE project
     2. Name: Label-free quantitative proteomics in Candida yeast species: technical and biological replicates to asses data reproducibility