PXD012354-2

PXD012354 is an original dataset announced via ProteomeXchange.

Title	GPIF constrains Polycomb Repressive Complex 2 activity in germ cells
Description	The project aimed at identifying the cofactors regulating Polycomb complex PRC2 enzymatic activity in gonads. We used a knock-in mouse model where the enzymatic subunit of PRC2 (either EZH2 or EZH1) is tagged (Flag-tag) to purify PRC2 from mouse adult testis. This experiment revealed the existence of a new cofactor for PRC2 that we called GPIF (AU022751).
HostingRepository	PRIDE
AnnounceDate	2019-07-22
AnnouncementXML	Submission_2019-08-30_00:43:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pascal Jansen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-15 07:36:37	ID requested
1	2019-07-22 09:07:23	announced
⏵ 2	2019-08-30 00:43:31	announced	Updated publication reference for PubMed record(s): 31451685.

Ragazzini R, P, é, rez-Palacios R, Baymaz IH, Diop S, Ancelin K, Zielinski D, Michaud A, Givelet M, Borsos M, Aflaki S, Legoix P, Jansen PWTC, Servant N, Torres-Padilla ME, Bourc'his D, Fouchet P, Vermeulen M, Margueron R, EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells. Nat Commun, 10(1):3858(2019) [pubmed]

curator keyword: Biological

submitter keyword: Chromatin, histone, methylation, Polycomb.

Raphaël Margueron
contact affiliation	Institut Curie, Paris Sciences et Lettres Research University, INSERM U934/ CNRS, UMR3215
contact email	raphael.margueron@curie.fr
lab head
Pascal Jansen
contact affiliation	Radboud University
contact email	p.jansen@science.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012354

PRIDE project URI

• PRIDE
  1. PXD012354
     1. Label: PRIDE project
     2. Name: GPIF constrains Polycomb Repressive Complex 2 activity in germ cells