PXD012106-3

PXD012106 is an original dataset announced via ProteomeXchange.

Title	The Trichoplax H2 holobiont: a simple animal and its two intracellular symbionts
Description	The Placozoa are an enigmatic group of simple marine metazoans where all taxa harbor intracellular bacteria. Despite four decades of research, the bacterial identities, their location and their roles remain elusive. Here we show that the placozoan Trichoplax H2 is associated with two intracellular bacteria. We detected the symbionts and reconstructed their physiology using metagenomic and metatranscriptomic evidence from the same single-animal specimens. One symbiont forms a new genus in the Midichloriaceae (Rickettsiales) and correlative fluorescent labelling and 3-D electron microscopic tomography showed that it inhabits the rough ER in the fiber-cells. It has mutualistic traits and occurs worldwide as we could detect it in 10% of all aquatic tag sequencing datasets. The second symbiont is an intracellular bacterium from the Margulisbacteria, a phylum-level clade previously only identified from DNA sequencing and not known to form intracellular associations. It resides in the digestive ventral epithelial cells, uses lipids digested by the host and has the physiological capacity to supplement the placozoan nutrition. Our single-individual approach revealed that this cultivable placozoan host forms a tripartite symbiosis and provides experimental access to microbial dark matter – a rickettsiales that inhabits a novel niche within eukaryote cells and an intracellular margulisbacterial symbiont.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:51:38.150.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tjorven Hinzke
SpeciesList	scientific name: Candidatus Midichloriaceae; NCBI TaxID: 1328881; scientific name: Trichoplax sp. H2; NCBI TaxID: 287889; scientific name: Candidatus Margulisbacteria; NCBI TaxID: 1817808;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-19 00:10:33	ID requested
1	2019-04-18 01:46:17	announced
2	2019-06-17 03:18:38	announced	Updated publication reference for PubMed record(s): 31182796.
⏵ 3	2024-10-22 04:51:40	announced	2024-10-22: Updated project metadata.

Gruber-Vodicka HR, Leisch N, Kleiner M, Hinzke T, Liebeke M, McFall-Ngai M, Hadfield MG, Dubilier N, Two intracellular and cell type-specific bacterial symbionts in the placozoan Trichoplax H2. Nat Microbiol, 4(9):1465-1474(2019) [pubmed]

10.1038/s41564-019-0475-9;

curator keyword: Biological

submitter keyword: Grellia incantans, rough endoplasmic reticulum, Trichoplax H2, Ruthmannia eludens,Placozoa, intracellular symbiosis, Midichloriaceae, Rickettsiales, Margulisbacteria

Manuel Kleiner
contact affiliation	Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina, USA
contact email	manuel_kleiner@ncsu.edu
lab head
Tjorven Hinzke
contact affiliation	University of Greifswald, Greifswald Intitute of Marine Biotechnology
contact email	tjorven.hinzke@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012106

PRIDE project URI

• PRIDE
  1. PXD012106
     1. Label: PRIDE project
     2. Name: The Trichoplax H2 holobiont: a simple animal and its two intracellular symbionts