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PXD012106 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Trichoplax H2 holobiont: a simple animal and its two intracellular symbionts
DescriptionThe Placozoa are an enigmatic group of simple marine metazoans where all taxa harbor intracellular bacteria. Despite four decades of research, the bacterial identities, their location and their roles remain elusive. Here we show that the placozoan Trichoplax H2 is associated with two intracellular bacteria. We detected the symbionts and reconstructed their physiology using metagenomic and metatranscriptomic evidence from the same single-animal specimens. One symbiont forms a new genus in the Midichloriaceae (Rickettsiales) and correlative fluorescent labelling and 3-D electron microscopic tomography showed that it inhabits the rough ER in the fiber-cells. It has mutualistic traits and occurs worldwide as we could detect it in 10% of all aquatic tag sequencing datasets. The second symbiont is an intracellular bacterium from the Margulisbacteria, a phylum-level clade previously only identified from DNA sequencing and not known to form intracellular associations. It resides in the digestive ventral epithelial cells, uses lipids digested by the host and has the physiological capacity to supplement the placozoan nutrition. Our single-individual approach revealed that this cultivable placozoan host forms a tripartite symbiosis and provides experimental access to microbial dark matter – a rickettsiales that inhabits a novel niche within eukaryote cells and an intracellular margulisbacterial symbiont.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTjorven Hinzke
SpeciesList scientific name: Candidatus Midichloriaceae; NCBI TaxID: 1328881; scientific name: Trichoplax sp. H2; NCBI TaxID: 287889; scientific name: Candidatus Margulisbacteria; NCBI TaxID: 1817808;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-12-19 00:10:33ID requested
12019-04-18 01:46:17announced
22019-06-17 03:18:38announcedUpdated publication reference for PubMed record(s): 31182796.
Publication List
Gruber-Vodicka HR, Leisch N, Kleiner M, Hinzke T, Liebeke M, McFall-Ngai M, Hadfield MG, Dubilier N, Two intracellular and cell type-specific bacterial symbionts in the placozoan Trichoplax H2. Nat Microbiol, 4(9):1465-1474(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Placozoa, Trichoplax H2, intracellular symbiosis, Rickettsiales, Grellia incantans, rough endoplasmic reticulum, Midichloriaceae, Ruthmannia eludens, Margulisbacteria
Contact List
Manuel Kleiner
contact affiliationDepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina, USA
contact emailmanuel_kleiner@ncsu.edu
lab head
Tjorven Hinzke
contact affiliationUniversity of Greifswald, Greifswald Intitute of Marine Biotechnology
contact emailtjorven.hinzke@stud.uni-greifswald.de
dataset submitter
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Dataset FTP location
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