PXD012041 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Response of the Biomining Acidithiobacillus ferrooxidans to high Cadmium Concentrations |
Description | Cadmium is one of several heavy metals present in contaminated soils. Apparently, it has no biological role but can produce DNA damage, overexpression of stress response proteins and misfolded proteins, amongst other deleterial effects. Acidithiobacillus ferrooxidans is an acidophilic bacterium capable of resisting very high concentrations of heavy metals such as cadmium. This is important for industrial bioleaching processes where Cd+2 concentrations can be in the range of 5-100 mM. Cadmium resistance mechanisms in these microorganisms have not been fully characterized. A. ferrooxidans ATCC 53993 contains genes coding for possible metal resistance determinants such as efflux systems belonging to three families: P-type ATPases, RND transporters and cation diffusion facilitators (CDF). In addition, it has some extra copies of these genes in its exclusive genomic island (GI). Several of these putative genes were characterized in the present report by determining their transcriptional expression profiles and functionality. Moreover, a global quantitative proteomic analysis was carried out to further explore new cadmium resistance determinants in this biomining acidophile. Changes in iron oxidation pathways, upregulation of transport proteins (P-type ATPases and CDFs) and changes in ribosomal protein levels were seen. Finally, increased concentrations of exclusive putative cadmium ATPases present in strain ATCC 53993 GI and other non-identified proteins such as Lferr_0210, which forms part of a possible operon, could explain its greater resistance to cadmium compared to other acidophiles such as A. ferrooxidans ATCC 23270. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:34:16.356.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alberto Paradela |
SpeciesList | scientific name: Acidithiobacillus ferrooxidans ATCC 53993; NCBI TaxID: 380394; |
ModificationList | iTRAQ4plex-116 reporter+balance reagent acylated residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-12-13 01:09:37 | ID requested | |
1 | 2022-02-28 09:00:52 | announced | |
⏵ 2 | 2024-10-22 05:34:16 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Acidithiobacillus ferrooxidans |
Cadmium |
quantitative proteomics |
transcriptomics |
Contact List
Carlos Jerez |
contact affiliation | Laboratory of Molecular Microbiology and Biotechnology, Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile. |
contact email | cjerez@uchile.cl |
lab head | |
Alberto Paradela |
contact affiliation | Proteomics Lab CNB-CSIC |
contact email | alberto.paradela@cnb.csic.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012041
- Label: PRIDE project
- Name: Response of the Biomining Acidithiobacillus ferrooxidans to high Cadmium Concentrations