PXD012041-1

PXD012041 is an original dataset announced via ProteomeXchange.

Title	Response of the Biomining Acidithiobacillus ferrooxidans to high Cadmium Concentrations
Description	Cadmium is one of several heavy metals present in contaminated soils. Apparently, it has no biological role but can produce DNA damage, overexpression of stress response proteins and misfolded proteins, amongst other deleterial effects. Acidithiobacillus ferrooxidans is an acidophilic bacterium capable of resisting very high concentrations of heavy metals such as cadmium. This is important for industrial bioleaching processes where Cd+2 concentrations can be in the range of 5-100 mM. Cadmium resistance mechanisms in these microorganisms have not been fully characterized. A. ferrooxidans ATCC 53993 contains genes coding for possible metal resistance determinants such as efflux systems belonging to three families: P-type ATPases, RND transporters and cation diffusion facilitators (CDF). In addition, it has some extra copies of these genes in its exclusive genomic island (GI). Several of these putative genes were characterized in the present report by determining their transcriptional expression profiles and functionality. Moreover, a global quantitative proteomic analysis was carried out to further explore new cadmium resistance determinants in this biomining acidophile. Changes in iron oxidation pathways, upregulation of transport proteins (P-type ATPases and CDFs) and changes in ribosomal protein levels were seen. Finally, increased concentrations of exclusive putative cadmium ATPases present in strain ATCC 53993 GI and other non-identified proteins such as Lferr_0210, which forms part of a possible operon, could explain its greater resistance to cadmium compared to other acidophiles such as A. ferrooxidans ATCC 23270.
HostingRepository	PRIDE
AnnounceDate	2022-02-28
AnnouncementXML	Submission_2022-02-28_09:00:52.635.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alberto Paradela
SpeciesList	scientific name: Acidithiobacillus ferrooxidans ATCC 53993; NCBI TaxID: 380394;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-13 01:09:37	ID requested
⏵ 1	2022-02-28 09:00:52	announced
2	2024-10-22 05:34:16	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Acidithiobacillus ferrooxidans

Cadmium

quantitative proteomics

transcriptomics

Carlos Jerez
contact affiliation	Laboratory of Molecular Microbiology and Biotechnology, Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile.
contact email	cjerez@uchile.cl
lab head
Alberto Paradela
contact affiliation	CNB-CSIC
contact email	alberto.paradela@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012041
     1. Label: PRIDE project
     2. Name: Response of the Biomining Acidithiobacillus ferrooxidans to high Cadmium Concentrations