PXD010831 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MIR sequences recruit zinc finger protein ZNF768 to expressed genes |
Description | Mammalian-wide interspersed repeats (MIRs) are retrotransposed elements of mammalian genomes. Here we report the specific binding of zinc finger protein ZNF768 to the sequence motif GCTGTGTG (N20) CCTCTCTG in the core region of MIRs. ZNF768 binding is preferentially associated with euchromatin and promoter regions of genes. Binding was observed for genes expressed in a cell type-specific manner in human B cell line Raji and osteosarcoma U2OS cells. Mass spectrometric analysis of ZNF768 associated proteins revealed binding of ZNF768 to Elongator subunits 1, 2, and 3. The N-terminus of ZNF768 contains a heptad repeat array structurally related to the C-terminal domain (CTD) of RNA polymerase II. This array evolved in placental animals but not marsupials and monotreme species, displays species-specific length variations, and possibly fulfills CTD related functions in gene regulation. We propose that the evolution of MIRs and ZNF768 has extended the repertoire of gene regulatory mechanisms in mammals and that ZNF768 binding is associated with cell type-specific gene expression. |
HostingRepository | PRIDE |
AnnounceDate | 2018-10-29 |
AnnouncementXML | Submission_2018-12-03_02:19:02.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ignasi Forne |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-21 02:12:33 | ID requested | |
1 | 2018-10-29 05:58:30 | announced | |
⏵ 2 | 2018-12-03 02:19:04 | announced | Updated publication reference for PubMed record(s): 30476274. |
3 | 2018-12-04 03:58:53 | announced | Updated project metadata. |
Publication List
Rohrmoser M, Kluge M, Yahia Y, Gruber-Eber A, Maqbool MA, Forn, é I, Krebs S, Blum H, Greifenberg AK, Geyer M, Descostes N, Imhof A, Andrau JC, Friedel CC, Eick D, MIR sequences recruit zinc finger protein ZNF768 to expressed genes. Nucleic Acids Res, 47(2):700-715(2019) [pubmed] |
Keyword List
submitter keyword: Zinc finger protein, heptad-repeats, mammalian interspersed repeats, gene regulation, placentalia, Elongator |
Contact List
Prof. Dr. Dirk Eick |
contact affiliation | Research Unit Molecular Epigenetics Head of the Research unit Helmholtz Zentrum München German Research Center for Environmental Health (GmbH) Research Unit Molecular Epigenetics Marchioninistraße 25 81377 Munich Phone: +49 89 3187-1512 E-mail: eick@helmholtz-muenchen.de |
contact email | eick@helmholtz-muenchen.de |
lab head | |
Ignasi Forne |
contact affiliation | Biomedical Center-LMU |
contact email | ignasi.forne@lrz.uni-muenchen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010831
- Label: PRIDE project
- Name: MIR sequences recruit zinc finger protein ZNF768 to expressed genes