PXD010831-1

PXD010831 is an original dataset announced via ProteomeXchange.

Title	MIR sequences recruit zinc finger protein ZNF768 to expressed genes
Description	Mammalian-wide interspersed repeats (MIRs) are retrotransposed elements of mammalian genomes. Here we report the specific binding of zinc finger protein ZNF768 to the sequence motif GCTGTGTG (N20) CCTCTCTG in the core region of MIRs. ZNF768 binding is preferentially associated with euchromatin and promoter regions of genes. Binding was observed for genes expressed in a cell type-specific manner in human B cell line Raji and osteosarcoma U2OS cells. Mass spectrometric analysis of ZNF768 associated proteins revealed binding of ZNF768 to Elongator subunits 1, 2, and 3. The N-terminus of ZNF768 contains a heptad repeat array structurally related to the C-terminal domain (CTD) of RNA polymerase II. This array evolved in placental animals but not marsupials and monotreme species, displays species-specific length variations, and possibly fulfills CTD related functions in gene regulation. We propose that the evolution of MIRs and ZNF768 has extended the repertoire of gene regulatory mechanisms in mammals and that ZNF768 binding is associated with cell type-specific gene expression.
HostingRepository	PRIDE
AnnounceDate	2018-10-29
AnnouncementXML	Submission_2018-10-29_05:58:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-21 02:12:33	ID requested
⏵ 1	2018-10-29 05:58:30	announced
2	2018-12-03 02:19:04	announced	Updated publication reference for PubMed record(s): 30476274.
3	2018-12-04 03:58:53	announced	Updated project metadata.

Dataset with its publication pending

submitter keyword: Zinc finger protein, heptad-repeats, mammalian interspersed repeats, gene regulation, placentalia, Elongator

Prof. Dr. Dirk Eick
contact affiliation	Research Unit Molecular Epigenetics Head of the Research unit Helmholtz Zentrum München German Research Center for Environmental Health (GmbH) Research Unit Molecular Epigenetics Marchioninistraße 25 81377 Munich Phone: +49 89 3187-1512 E-mail: eick@helmholtz-muenchen.de
contact email	eick@helmholtz-muenchen.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD010831

PRIDE project URI

• PRIDE
  1. PXD010831
     1. Label: PRIDE project
     2. Name: MIR sequences recruit zinc finger protein ZNF768 to expressed genes