PXD010830-3
PXD010830 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome of Medusavirus |
Description | Medusavirus particles was resuspended in PBS containing 0.5% SDS and protease inhibitor cocktail (Nacalai Tesque, Japan) and incubated for 1 h at 65°C. Samples were subjected to trichloroacetic acid (TCA) precipitation, followed by protein quantification using the BCA method. Proteins were reduced for 2 h at 37°C, and subsequently alkylated using iodoacetamide for 30 min at 20 to 25°C (room temperature), followed by treatment with trypsin for 20 h at 37°C. After desalination and concentration, the treated proteins were subjected to LC–MS/MS analysis using EASY-nLC 1200 (Thermo Fisher Scientific Inc., USA) and Q Exactive Plus (Thermo Fisher Scientific Inc., USA). |
HostingRepository | jPOST |
AnnounceDate | 2019-01-30 |
AnnouncementXML | Submission_2022-09-18_03:55:12.464.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Genki Yoshikawa |
SpeciesList | scientific name: cellular organisms; NCBI TaxID: 131567; |
ModificationList | unknown modification; L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2018-08-21 00:05:50 | ID requested | |
1 | 2019-01-29 17:29:17 | announced | |
2 | 2021-02-04 16:51:00 | announced | 2021-02-04: Updated FTP location. |
⏵ 3 | 2022-09-18 03:55:12 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Medusavirus, purified particles, LC-MS/MS |
Contact List
Masaharu Takemura | |
---|---|
lab head | |
Genki Yoshikawa | |
contact affiliation | Kyoto University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000467/ |