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PXD010830-3

PXD010830 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome of Medusavirus
DescriptionMedusavirus particles was resuspended in PBS containing 0.5% SDS and protease inhibitor cocktail (Nacalai Tesque, Japan) and incubated for 1 h at 65°C. Samples were subjected to trichloroacetic acid (TCA) precipitation, followed by protein quantification using the BCA method. Proteins were reduced for 2 h at 37°C, and subsequently alkylated using iodoacetamide for 30 min at 20 to 25°C (room temperature), followed by treatment with trypsin for 20 h at 37°C. After desalination and concentration, the treated proteins were subjected to LC–MS/MS analysis using EASY-nLC 1200 (Thermo Fisher Scientific Inc., USA) and Q Exactive Plus (Thermo Fisher Scientific Inc., USA).
HostingRepositoryjPOST
AnnounceDate2019-01-30
AnnouncementXMLSubmission_2022-09-18_03:55:12.464.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGenki Yoshikawa
SpeciesList scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationListunknown modification; L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-08-21 00:05:50ID requested
12019-01-29 17:29:17announced
22021-02-04 16:51:00announced2021-02-04: Updated FTP location.
32022-09-18 03:55:12announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Medusavirus, purified particles, LC-MS/MS
Contact List
Masaharu Takemura
lab head
Genki Yoshikawa
contact affiliationKyoto University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000467/