PXD010830-2

PXD010830 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteome of Medusavirus
Description	Medusavirus particles was resuspended in PBS containing 0.5% SDS and protease inhibitor cocktail (Nacalai Tesque, Japan) and incubated for 1 h at 65°C. Samples were subjected to trichloroacetic acid (TCA) precipitation, followed by protein quantification using the BCA method. Proteins were reduced for 2 h at 37°C, and subsequently alkylated using iodoacetamide for 30 min at 20 to 25°C (room temperature), followed by treatment with trypsin for 20 h at 37°C. After desalination and concentration, the treated proteins were subjected to LC–MS/MS analysis using EASY-nLC 1200 (Thermo Fisher Scientific Inc., USA) and Q Exactive Plus (Thermo Fisher Scientific Inc., USA).
HostingRepository	jPOST
AnnounceDate	2019-01-30
AnnouncementXML	Submission_2021-02-04_16:50:59.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Genki Yoshikawa
SpeciesList	scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	unknown modification; L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-21 00:05:50	ID requested
1	2019-01-29 17:29:17	announced
⏵ 2	2021-02-04 16:51:00	announced	2021-02-04: Updated FTP location.
3	2022-09-18 03:55:12	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Medusavirus, purified particles, LC-MS/MS

submitter keyword: Medusavirus, purified particles, LC-MS/MS

Contact List

Masaharu Takemura
lab head
Genki Yoshikawa
contact affiliation	Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000467

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000467