PXD009968-3

PXD009968 is an original dataset announced via ProteomeXchange.

Title	AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy
Description	The AMP-activated protein kinase (AMPK) is a master sensor of the cellular energy status and is crucial for the adaptive response to limited energy availability. AMPK is implicated in the regulation of many cellular processes, including autophagy. However, the precise mechanisms by which AMPK controls these processes and the identities of relevant substrates are not fully understood. With a protein microarrays we identified Cyclin Y as novel AMPK substrate that is phosphorylated at S326 both in vitro and in cells. Phosphorylation of Cyclin Y at S326 promotes its interaction with the cyclin-dependent kinase 16 (CDK16), thereby stimulating its catalytic activity. When expressed in cells, Cyclin Y/CDK16 is sufficient to promote autophagy. Moreover, Cyclin Y/CDK16 is necessary for efficient AMPK-dependent activation of autophagy. This functional interaction is mediated by AMPK phosphorylating S326 of Cyclin Y. Collectively, we define Cyclin Y/CDK16 as downstream effector of AMPK for inducing autophagy.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:07:34.170.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Preisinger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-30 15:56:38	ID requested
1	2020-02-17 02:20:01	announced
2	2020-03-24 01:08:29	announced	2020-03-24: Updated publication reference for PubMed record(s): 32098961.
⏵ 3	2024-10-22 04:07:35	announced	2024-10-22: Updated project metadata.

Dohmen M, Krieg S, Agalaridis G, Zhu X, Shehata SN, Pfeiffenberger E, Amelang J, B, ü, tepage M, Buerova E, Pfaff CM, Chanda D, Geley S, Preisinger C, Sakamoto K, L, ü, scher B, Neumann D, Vervoorts J, AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy. Nat Commun, 11(1):1032(2020) [pubmed]

10.1038/s41467-020-14812-0;

curator keyword: Biomedical

submitter keyword: Human, Autophagy, nanoLC-MSMS, Phosphorylation

Jörg Vervoorts
contact affiliation	Institute of Biochemistry and Molecular Biology, Medical School, RWTH Aachen University, 52074 Aachen, Germany
contact email	jvervoorts-weber@ukaachen.de
lab head
Christian Preisinger
contact affiliation	Proteomics Facility, IZKF Aachen, University Clinic RWTH Aachen
contact email	cpreisinger@ukaachen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD009968

PRIDE project URI

• PRIDE
  1. PXD009968
     1. Label: PRIDE project
     2. Name: AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy