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PXD009968-1

PXD009968 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy
DescriptionThe AMP-activated protein kinase (AMPK) is a master sensor of the cellular energy status and is crucial for the adaptive response to limited energy availability. AMPK is implicated in the regulation of many cellular processes, including autophagy. However, the precise mechanisms by which AMPK controls these processes and the identities of relevant substrates are not fully understood. With a protein microarrays we identified Cyclin Y as novel AMPK substrate that is phosphorylated at S326 both in vitro and in cells. Phosphorylation of Cyclin Y at S326 promotes its interaction with the cyclin-dependent kinase 16 (CDK16), thereby stimulating its catalytic activity. When expressed in cells, Cyclin Y/CDK16 is sufficient to promote autophagy. Moreover, Cyclin Y/CDK16 is necessary for efficient AMPK-dependent activation of autophagy. This functional interaction is mediated by AMPK phosphorylating S326 of Cyclin Y. Collectively, we define Cyclin Y/CDK16 as downstream effector of AMPK for inducing autophagy.
HostingRepositoryPRIDE
AnnounceDate2020-02-17
AnnouncementXMLSubmission_2020-02-17_02:20:00.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristian Preisinger
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentQ Exactive; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-05-30 15:56:38ID requested
12020-02-17 02:20:01announced
22020-03-24 01:08:29announced2020-03-24: Updated publication reference for PubMed record(s): 32098961.
32024-10-22 04:07:35announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: Human, Autophagy, Phosphorylation, nanoLC-MSMS
Contact List
Jörg Vervoorts
contact affiliationInstitute of Biochemistry and Molecular Biology, Medical School, RWTH Aachen University, 52074 Aachen, Germany
contact emailjvervoorts-weber@ukaachen.de
lab head
Christian Preisinger
contact affiliationProteomics Facility, IZKF Aachen, University Clinic RWTH Aachen
contact emailcpreisinger@ukaachen.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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