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PXD009730-2

PXD009730 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePorphyromonas gingivalis Outer Membrane Vesicle and cell Proteome in heme excess and heme limited
DescriptionPorphyromonas gingivalis is an anaerobic, Gram-negative oral pathogen associated with chronic periodontitis. P. gingivalis has an obligate requirement for heme which it obtains from the host. Heme availability has been linked to disease initiation and progression. In this study we used continuous culture of the bacterium to determine the effect of heme limitation and excess on the P. gingivalis proteome. Four biological replicates of whole cell lysate (WCL) and outer membrane vesicle (OMV) samples were digested with trypsin and analysed by tandem mass spectrometry and MaxQuant label-free quantification. In total, 1211 proteins were quantified with 108 and 49 proteins significantly changing in abundance more than 1.5-fold (p<0.05) in the WCLs and OMVs respectively. The proteins most upregulated in response to heme limitation were those involved in binding and transporting heme while the four proteins most upregulated in the heme excess condition constitute a putative heme efflux system. In general, the protein abundance ratios obtained for OMVs and WCLs agreed, indicating that changes to the OM protein composition are passed on to OMVs, however 16 proteins were preferentially packaged into OMVs in one condition more than the other. In particular, moonlighting cytoplasmic proteins were preferentially associated with OMVs under heme excess.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:45:08.979.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPaul Veith
SpeciesList scientific name: Porphyromonas gingivalis W50; NCBI TaxID: 1125722;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-05-09 09:25:01ID requested
12018-05-18 03:42:51announced
22024-10-22 04:45:12announced2024-10-22: Updated project metadata.
Publication List
10.1021/acs.jproteome.8b00153;
Veith PD, Luong C, Tan KH, Dashper SG, Reynolds EC, Outer Membrane Vesicle Proteome of Porphyromonas gingivalis Is Differentially Modulated Relative to the Outer Membrane in Response to Heme Availability. J Proteome Res, 17(7):2377-2389(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Porphyromonas gingivalis, heme, MaxQuant, Outer Membrane Vesicles
Contact List
Eric Charles Reynolds
contact affiliationMelbourne Dental School, University of Melbourne, Australia
contact emaile.reynolds@unimelb.edu.au
lab head
Paul Veith
contact affiliationMelbourne Dental School
contact emailpdv@unimelb.edu.au
dataset submitter
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