PXD009730 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Porphyromonas gingivalis Outer Membrane Vesicle and cell Proteome in heme excess and heme limited |
Description | Porphyromonas gingivalis is an anaerobic, Gram-negative oral pathogen associated with chronic periodontitis. P. gingivalis has an obligate requirement for heme which it obtains from the host. Heme availability has been linked to disease initiation and progression. In this study we used continuous culture of the bacterium to determine the effect of heme limitation and excess on the P. gingivalis proteome. Four biological replicates of whole cell lysate (WCL) and outer membrane vesicle (OMV) samples were digested with trypsin and analysed by tandem mass spectrometry and MaxQuant label-free quantification. In total, 1211 proteins were quantified with 108 and 49 proteins significantly changing in abundance more than 1.5-fold (p<0.05) in the WCLs and OMVs respectively. The proteins most upregulated in response to heme limitation were those involved in binding and transporting heme while the four proteins most upregulated in the heme excess condition constitute a putative heme efflux system. In general, the protein abundance ratios obtained for OMVs and WCLs agreed, indicating that changes to the OM protein composition are passed on to OMVs, however 16 proteins were preferentially packaged into OMVs in one condition more than the other. In particular, moonlighting cytoplasmic proteins were preferentially associated with OMVs under heme excess. |
HostingRepository | PRIDE |
AnnounceDate | 2018-05-18 |
AnnouncementXML | Submission_2018-05-18_03:42:49.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Paul Veith |
SpeciesList | scientific name: Porphyromonas gingivalis W50; NCBI TaxID: 1125722; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-05-09 09:25:01 | ID requested | |
⏵ 1 | 2018-05-18 03:42:51 | announced | |
2 | 2024-10-22 04:45:12 | announced | 2024-10-22: Updated project metadata. |
Publication List
Veith PD, Luong C, Tan KH, Dashper SG, Reynolds EC, Outer Membrane Vesicle Proteome of Porphyromonas gingivalis Is Differentially Modulated Relative to the Outer Membrane in Response to Heme Availability. J Proteome Res, 17(7):2377-2389(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Porphyromonas gingivalis, MaxQuant, Outer Membrane Vesicles, heme |
Contact List
Eric Charles Reynolds |
contact affiliation | Melbourne Dental School, University of Melbourne, Australia |
contact email | e.reynolds@unimelb.edu.au |
lab head | |
Paul Veith |
contact affiliation | Melbourne Dental School |
contact email | pdv@unimelb.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009730
- Label: PRIDE project
- Name: Porphyromonas gingivalis Outer Membrane Vesicle and cell Proteome in heme excess and heme limited