PXD009730-1

PXD009730 is an original dataset announced via ProteomeXchange.

Title	Porphyromonas gingivalis Outer Membrane Vesicle and cell Proteome in heme excess and heme limited
Description	Porphyromonas gingivalis is an anaerobic, Gram-negative oral pathogen associated with chronic periodontitis. P. gingivalis has an obligate requirement for heme which it obtains from the host. Heme availability has been linked to disease initiation and progression. In this study we used continuous culture of the bacterium to determine the effect of heme limitation and excess on the P. gingivalis proteome. Four biological replicates of whole cell lysate (WCL) and outer membrane vesicle (OMV) samples were digested with trypsin and analysed by tandem mass spectrometry and MaxQuant label-free quantification. In total, 1211 proteins were quantified with 108 and 49 proteins significantly changing in abundance more than 1.5-fold (p<0.05) in the WCLs and OMVs respectively. The proteins most upregulated in response to heme limitation were those involved in binding and transporting heme while the four proteins most upregulated in the heme excess condition constitute a putative heme efflux system. In general, the protein abundance ratios obtained for OMVs and WCLs agreed, indicating that changes to the OM protein composition are passed on to OMVs, however 16 proteins were preferentially packaged into OMVs in one condition more than the other. In particular, moonlighting cytoplasmic proteins were preferentially associated with OMVs under heme excess.
HostingRepository	PRIDE
AnnounceDate	2018-05-18
AnnouncementXML	Submission_2018-05-18_03:42:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Veith
SpeciesList	scientific name: Porphyromonas gingivalis W50; NCBI TaxID: 1125722;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-09 09:25:01	ID requested
⏵ 1	2018-05-18 03:42:51	announced
2	2024-10-22 04:45:12	announced	2024-10-22: Updated project metadata.

curator keyword: Biological

submitter keyword: Porphyromonas gingivalis, MaxQuant, Outer Membrane Vesicles, heme

Eric Charles Reynolds
contact affiliation	Melbourne Dental School, University of Melbourne, Australia
contact email	e.reynolds@unimelb.edu.au
lab head
Paul Veith
contact affiliation	Melbourne Dental School
contact email	pdv@unimelb.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009730
     1. Label: PRIDE project
     2. Name: Porphyromonas gingivalis Outer Membrane Vesicle and cell Proteome in heme excess and heme limited