PXD009146-4
PXD009146 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | K562 Chemical arrest cell cycle phase fractions 2D-MS-CETSA |
Description | K562 cells were synchronized by double thymidine block(DTB) based chemical arrest scheme into G1/S, S and Prometaphase(PM) fractions. Three biological replicates were collected on different days, and assembled into a 2D-CETSA scheme with 6 different heating temperatures (37ºC, 47ºC, 50ºC, 52ºC,54ºC and 57ºC), (Figure 1A, Methods section of the associated paper), with each set of samples heated at one temperature included in the same isobaric TMT10 set and measured at the same time on Mass Spectrometer. The labeling order of samples in the TMT10 channels is as follows: 37C/47C/50C/52C/54C/57C samples: Biorep1_G1S(126), Biorep1_S(127N), Biorep1_PM(127C), Biorep2_G1S(128N), Biorep2_S(128C), Biorep2_PM(129N), Biorep3_G1S(129C), Biorep3_S(130N), Biorep3_PM(130C), mixed_control(131) |
HostingRepository | jPOST |
AnnounceDate | 2018-05-08 |
AnnouncementXML | Submission_2022-09-18_02:15:14.127.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Radoslaw Sobota |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent N6-acylated lysine; L-methionine sulfoxide; TMT6plex-126 reporter+balance reagent acylated N-terminal; S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; deamidated L-asparagine; deamidated L-glutamine |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2018-03-08 17:53:34 | ID requested | |
1 | 2018-05-07 08:00:10 | announced | |
2 | 2018-09-19 00:56:15 | announced | Updated CV, FTP location and Modification list. |
3 | 2021-02-04 16:57:10 | announced | 2021-02-04: Updated FTP location. |
⏵ 4 | 2022-09-18 02:15:14 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dai L, Zhao T, Bisteau X, Sun W, Prabhu N, Lim YT, Sobota RM, Kaldis P, Nordlund P, Modulation of Protein-Interaction States through the Cell Cycle. Cell, 173(6):1481-1494.e13(2018) [pubmed] |
Keyword List
submitter keyword: MS-CETSA, cell cycle, Protein interaction state, chemical arrest |
Contact List
Pär Nordlund | |
---|---|
lab head | |
Radoslaw Sobota | |
contact affiliation | IMCB A-STAR Singapore |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000401/ |