PXD009146-3

PXD009146 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	K562 Chemical arrest cell cycle phase fractions 2D-MS-CETSA
Description	K562 cells were synchronized by double thymidine block(DTB) based chemical arrest scheme into G1/S, S and Prometaphase(PM) fractions. Three biological replicates were collected on different days, and assembled into a 2D-CETSA scheme with 6 different heating temperatures (37ºC, 47ºC, 50ºC, 52ºC,54ºC and 57ºC), (Figure 1A, Methods section of the associated paper), with each set of samples heated at one temperature included in the same isobaric TMT10 set and measured at the same time on Mass Spectrometer. The labeling order of samples in the TMT10 channels is as follows: 37C/47C/50C/52C/54C/57C samples: Biorep1_G1S(126), Biorep1_S(127N), Biorep1_PM(127C), Biorep2_G1S(128N), Biorep2_S(128C), Biorep2_PM(129N), Biorep3_G1S(129C), Biorep3_S(130N), Biorep3_PM(130C), mixed_control(131)
HostingRepository	jPOST
AnnounceDate	2018-05-08
AnnouncementXML	Submission_2021-02-04_16:57:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Radoslaw Sobota
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent N6-acylated lysine; L-methionine sulfoxide; TMT6plex-126 reporter+balance reagent acylated N-terminal; S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; deamidated L-asparagine; deamidated L-glutamine
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-08 17:53:34	ID requested
1	2018-05-07 08:00:10	announced
2	2018-09-19 00:56:15	announced	Updated CV, FTP location and Modification list.
⏵ 3	2021-02-04 16:57:10	announced	2021-02-04: Updated FTP location.
4	2022-09-18 02:15:14	announced	2022-09-18: Updated FTP location.

Publication List

Dai L, Zhao T, Bisteau X, Sun W, Prabhu N, Lim YT, Sobota RM, Kaldis P, Nordlund P, Modulation of Protein-Interaction States through the Cell Cycle. Cell, 173(6):1481-1494.e13(2018) [pubmed]

Dai L, Zhao T, Bisteau X, Sun W, Prabhu N, Lim YT, Sobota RM, Kaldis P, Nordlund P, Modulation of Protein-Interaction States through the Cell Cycle. Cell, 173(6):1481-1494.e13(2018) [pubmed]

Keyword List

submitter keyword: MS-CETSA, cell cycle, Protein interaction state, chemical arrest

submitter keyword: MS-CETSA, cell cycle, Protein interaction state, chemical arrest

Contact List

Pär Nordlund
lab head
Radoslaw Sobota
contact affiliation	IMCB A-STAR Singapore
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000401