PXD008890-2

PXD008890 is an original dataset announced via ProteomeXchange.

Title	Retinal-cysteine adduct in Opn5L1
Description	Targeted analysis of retinal-cysteine adduct in Opsin 5 like-1 (Opn5L1) protein Chicken Opn5L1 E177K/Q192K mutant was expressed in HEK293T, regenerated by all-trans-retinal, solubilized by dodecyl maltoside, and purified using Rho1D4 antibody-conjugated Sephalose. Semi-quantitative targeted analyses of trypsinized Opn5L1 with or without light irradiation were carried out using reverse phase LC-ESI-MSn to detect light-dependent formation of retinal-cysteine adduct. The results were analyzed by Mass++, mMass and Igor Pro softwares.
HostingRepository	jPOST
AnnounceDate	2018-02-15
AnnouncementXML	Submission_2018-05-19_23:40:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keita Sato
SpeciesList	scientific name: Gallus gallus; NCBI TaxID: 9031;
ModificationList	iodoacetamide derivatized residue; unknown modification: Retinaloxime
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-07 23:32:23	ID requested
1	2018-02-14 07:00:10	announced
⏵ 2	2018-05-19 23:40:13	announced	Updated PubMed.
3	2018-09-19 00:48:23	announced	Updated CV, FTP location and Modification list.
4	2021-02-04 16:57:50	announced	2021-02-04: Updated FTP location.
5	2022-09-18 02:20:17	announced	2022-09-18: Updated FTP location.

Sato K, Yamashita T, Ohuchi H, Takeuchi A, Gotoh H, Ono K, Mizuno M, Mizutani Y, Tomonari S, Sakai K, Imamoto Y, Wada A, Shichida Y, Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor. Nat Commun, 9(1):1255(2018) [pubmed]

submitter keyword: label-free, targeted analysis

Yoshinori Shichida
lab head
Keita Sato
contact affiliation	Okayama University
dataset submitter

jPOST dataset URI