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PXD008890-1

PXD008890 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Retinal-cysteine adduct in Opn5L1
Description	Targeted analysis of retinal-cysteine adduct in Opsin 5 like-1 (Opn5L1) protein Chicken Opn5L1 E177K/Q192K mutant was expressed in HEK293T, regenerated by all-trans-retinal, solubilized by dodecyl maltoside, and purified using Rho1D4 antibody-conjugated Sephalose. Semi-quantitative targeted analyses of trypsinized Opn5L1 with or without light irradiation were carried out using reverse phase LC-ESI-MSn to detect light-dependent formation of retinal-cysteine adduct. The results were analyzed by Mass++, mMass and Igor Pro softwares.
HostingRepository	jPOST
AnnounceDate	2018-02-15
AnnouncementXML	Submission_2018-02-14_07:00:09.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keita Sato
SpeciesList	scientific name: Gallus gallus; NCBI TaxID: 9031;
ModificationList	iodoacetamide derivatized residue; unknown modification: Retinaloxime
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-07 23:32:23	ID requested
⏵ 1	2018-02-14 07:00:10	announced
2	2018-05-19 23:40:13	announced	Updated PubMed.
3	2018-09-19 00:48:23	announced	Updated CV, FTP location and Modification list.
4	2021-02-04 16:57:50	announced	2021-02-04: Updated FTP location.
5	2022-09-18 02:20:17	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: label-free, targeted analysis

Contact List

Yoshinori Shichida
lab head
Keita Sato
contact affiliation	Okayama University
dataset submitter

Full Dataset Link List

jPOST dataset URI