PXD008705-1

PXD008705 is an original dataset announced via ProteomeXchange.

Title	DiPIUS_Fbxw7
Description	Although elucidation of enzyme–substrate relations is fundamental to the advancement of biology, universal approaches to the identification of substrates for a given enzyme have not been established. It is especially difficult to identify substrates for ubiquitin ligases, given that most such substrates are immediately ubiquitylated and degraded as a result of their association with the enzyme. We here describe the development of a new approach, DiPIUS (differential proteomics-based identification of ubiquitylation substrates), to the discovery of substrates for ubiquitin ligases. We applied DiPIUS to Fbxw7α, and identified candidate substrates including previously known targets. DiPIUS is thus a powerful tool for unbiased and comprehensive screening for substrates of ubiquitin ligases.
HostingRepository	jPOST
AnnounceDate	2018-03-06
AnnouncementXML	Submission_2018-03-06_00:35:29.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kanae Yumimoto
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue; (2S,3R)-3-hydroxyaspartic acid; 2-pyrrolidone-5-carboxylic acid (Gln)
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-17 17:14:06	ID requested
⏵ 1	2018-03-06 00:35:30	announced
2	2018-09-19 00:45:36	announced	Updated CV, FTP location and Modification list.
3	2021-02-04 17:00:45	announced	2021-02-04: Updated FTP location.
4	2022-09-18 03:39:02	announced	2022-09-18: Updated FTP location.

Dataset with its publication pending

submitter keyword: substrates, Fbxw7, ubiquitin ligase

Keiichi I. Nakayama
lab head
Kanae Yumimoto
contact affiliation	Kyushu University
dataset submitter

jPOST dataset URI