PXD007988 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | T-cell synaptic ectosomes relay signals through microcluster transfer |
Description | Extracellular vesicles (EV) are proposed to transfer information between cells. In the immunological synapse T cell receptor (TCR) interaction with peptide-MHC drives microcluster formation and signaling that is terminated in part through sorting of TCR into EVs that bud into the synapse, synaptic ectosomes (SE). SE isolated on bead supported lipid bilayers (BSLB) contained TCR and a number of EV/retrovirus markers including CD317, a retroviral restriction factor. CD278 and CD154 were enriched in SE by respective ligands, expanding potential functions. Mass Spec profiling of SE identified endosomal sorting complexes required for transport (ESCRT) machinery and TCR microcluster components. Stochastic reconstruction microscopy (STORM) revealed TCR colocalization with CD278 and CD317, but segregation from CD154 within single SE. SE activated dendritic cells (DC) and CD154 recapitulated this effect only when presented on liposomes at SE density. |
HostingRepository | PRIDE |
AnnounceDate | 2019-09-12 |
AnnouncementXML | Submission_2019-09-12_01:39:48.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Roman Fischer |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-10-18 02:09:04 | ID requested | |
⏵ 1 | 2019-09-12 01:55:15 | announced | |
2 | 2019-10-02 00:59:01 | announced | 2019-10-02: Updated publication reference for PubMed record(s): 31469364. |
Publication List
Saliba DG, C, é, spedes-Donoso PF, B, á, lint Š, Compeer EB, Korobchevskaya K, Valvo S, Mayya V, Kvalvaag A, Peng Y, Dong T, Tognoli ML, O'Neill E, Bonham S, Fischer R, Kessler BM, Dustin ML, Composition and structure of synaptic ectosomes exporting antigen receptor linked to functional CD40 ligand from helper T cells. Elife, 8():(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Exosomes |
Contact List
Roman Fischer |
contact affiliation | Target Discovery Institute, University of Oxford |
contact email | roman.fischer@ndm.ox.ac.uk |
lab head | |
Roman Fischer |
contact affiliation | University of Oxford |
contact email | roman.fischer@ndm.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD007988 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007988
- Label: PRIDE project
- Name: T-cell synaptic ectosomes relay signals through microcluster transfer