Extracellular vesicles (EV) are proposed to transfer information between cells. In the immunological synapse T cell receptor (TCR) interaction with peptide-MHC drives microcluster formation and signaling that is terminated in part through sorting of TCR into EVs that bud into the synapse, synaptic ectosomes (SE). SE isolated on bead supported lipid bilayers (BSLB) contained TCR and a number of EV/retrovirus markers including CD317, a retroviral restriction factor. CD278 and CD154 were enriched in SE by respective ligands, expanding potential functions. Mass Spec profiling of SE identified endosomal sorting complexes required for transport (ESCRT) machinery and TCR microcluster components. Stochastic reconstruction microscopy (STORM) revealed TCR colocalization with CD278 and CD317, but segregation from CD154 within single SE. SE activated dendritic cells (DC) and CD154 recapitulated this effect only when presented on liposomes at SE density.