PXD007848-3
PXD007848 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | GFP-Trap-IP-phospho |
Description | To analyze phosphorylation sites of ALLO-1, worm eggs expressing GFP-ALLO-1 in the atg-11 or ikke-1 background were lysed in co-IP buffer, and GFP-ALLO-1 was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were collected, acidified to pH2.5 and desalted using GL-Tip SDB followed by LC-MS/MS analysis. |
HostingRepository | jPOST |
AnnounceDate | 2018-01-31 |
AnnouncementXML | Submission_2021-02-04_16:39:13.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hidetaka Kosako |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | unknown modification; L-methionine sulfoxide |
Instrument | instrument |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2017-09-27 01:16:06 | ID requested | |
1 | 2018-01-30 22:05:46 | announced | |
2 | 2018-09-19 00:37:06 | announced | Updated CV, FTP location and Modification list. |
⏵ 3 | 2021-02-04 16:39:14 | announced | 2021-02-04: Updated FTP location. |
4 | 2022-09-18 02:44:55 | announced | 2022-09-18: Updated FTP location. |
Publication List
Sato M, Sato K, Tomura K, Kosako H, Sato K, The autophagy receptor ALLO-1 and the IKKE-1 kinase control clearance of paternal mitochondria in Caenorhabditis elegans. Nat Cell Biol, 20(1):81-91(2018) [pubmed] |
Keyword List
submitter keyword: GFP-Trap |
Contact List
Hidetaka Kosako | |
---|---|
lab head | |
Hidetaka Kosako | |
contact affiliation | Tokushima University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000328 |