PXD007848-1
PXD007848 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | GFP-Trap-IP-phospho |
Description | To analyze phosphorylation sites of ALLO-1, worm eggs expressing GFP-ALLO-1 in the atg-11 or ikke-1 background were lysed in co-IP buffer, and GFP-ALLO-1 was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were collected, acidified to pH2.5 and desalted using GL-Tip SDB followed by LC-MS/MS analysis. |
HostingRepository | jPOST |
AnnounceDate | 2018-01-31 |
AnnouncementXML | Submission_2018-01-30_22:05:45.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hidetaka Kosako |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | unknown modification: none; (2S,3R)-3-hydroxyaspartic acid |
Instrument | instrument: Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2017-09-27 01:16:06 | ID requested | |
⏵ 1 | 2018-01-30 22:05:46 | announced | |
2 | 2018-09-19 00:37:06 | announced | Updated CV, FTP location and Modification list. |
3 | 2021-02-04 16:39:14 | announced | 2021-02-04: Updated FTP location. |
4 | 2022-09-18 02:44:55 | announced | 2022-09-18: Updated FTP location. |
Publication List
Sato M, Sato K, Tomura K, Kosako H, Sato K, The autophagy receptor ALLO-1 and the IKKE-1 kinase control clearance of paternal mitochondria in Caenorhabditis elegans. Nat Cell Biol, 20(1):81-91(2018) [pubmed] |
Keyword List
submitter keyword: GFP-Trap |
Contact List
Hidetaka Kosako | |
---|---|
lab head | |
Hidetaka Kosako | |
contact affiliation | Tokushima University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |