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PXD007848-1

PXD007848 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGFP-Trap-IP-phospho
DescriptionTo analyze phosphorylation sites of ALLO-1, worm eggs expressing GFP-ALLO-1 in the atg-11 or ikke-1 background were lysed in co-IP buffer, and GFP-ALLO-1 was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were collected, acidified to pH2.5 and desalted using GL-Tip SDB followed by LC-MS/MS analysis.
HostingRepositoryjPOST
AnnounceDate2018-01-31
AnnouncementXMLSubmission_2018-01-30_22:05:45.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHidetaka Kosako
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListunknown modification: none; (2S,3R)-3-hydroxyaspartic acid
Instrumentinstrument: Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-09-27 01:16:06ID requested
12018-01-30 22:05:46announced
22018-09-19 00:37:06announcedUpdated CV, FTP location and Modification list.
32021-02-04 16:39:14announced2021-02-04: Updated FTP location.
42022-09-18 02:44:55announced2022-09-18: Updated FTP location.
Publication List
Sato M, Sato K, Tomura K, Kosako H, Sato K, The autophagy receptor ALLO-1 and the IKKE-1 kinase control clearance of paternal mitochondria in Caenorhabditis elegans. Nat Cell Biol, 20(1):81-91(2018) [pubmed]
Keyword List
submitter keyword: GFP-Trap
Contact List
Hidetaka Kosako
lab head
Hidetaka Kosako
contact affiliationTokushima University
dataset submitter
Full Dataset Link List
jPOST dataset URI