PXD007821 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics study of Amblyomma variegatum saliva, characterization of the molecular determinants. |
Description | Amblyomma variegatum, the tropical bont tick, is a species of veterinary importance being considered as one of major pest of ruminants in Africa and in the Caribbean. It is associated with severe dermatophilosis and transmits the rickettsiale Ehrlichia ruminantium, the agent of heartwater, to ruminants. Tick’s saliva contains a complex mixture of bioactive molecules including proteins that modulate host responses to ensure successful blood feeding. The limited amount of saliva that can be obtained from ticks has hampered characterisation of salivary proteins using traditional protein chemistry, but recent advances in proteomics (especially mass spectrometry and bioinformatics) provide new tools to finely investigate tick sialomes. The general objective of this study was to better understand the role of A. variegatum saliva in tick/host/pathogen interactions. Salivation of semi-fed females was induced after detachment by injection of pilocarpin. Crude saliva proteins were pre-fractioned by SDS-PAGE and trypsin-generated peptides were analysed online by nano-flow Ultimate 3000 HPLC–nanoelectrospray ionization using an Orbitrap Elite mass spectrometer. Raw data analysis was performed using the MaxQuant software and proteins identified according to UniProt Knowledgebase. Our results bring new insights for a better understanding of tick-ruminant interactions at the molecular level, and pave the way towards integrative strategies to interfere with both the immunomosuppressive and infectious processes in corresponding tick-borne diseases. |
HostingRepository | PRIDE |
AnnounceDate | 2018-01-05 |
AnnouncementXML | Submission_2018-03-07_23:57:11.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Oana Vigy |
SpeciesList | scientific name: Amblyomma; NCBI TaxID: 6942; scientific name: Ixodes scapularis (Black-legged tick) (Deer tick); NCBI TaxID: 6945; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-09-22 08:24:30 | ID requested | |
1 | 2018-01-05 01:42:28 | announced | |
⏵ 2 | 2018-03-07 23:57:12 | announced | Updated publication reference for PubMed record(s): 29354598. |
3 | 2024-10-22 04:40:56 | announced | 2024-10-22: Updated project metadata. |
Publication List
Rodrigues V, Fernandez B, Vercoutere A, Chamayou L, Andersen A, Vigy O, Demettre E, Seveno M, Aprelon R, Giraud-Girard K, Stachurski F, Loire E, Vachi, é, ry N, Holzmuller P, Saliva on Bovine Cells: Characterization of Cellular Responses and Identification of Molecular Determinants. Front Cell Infect Microbiol, 7():521(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Amblyomma variegatum, tick saliva, immunomodulation, proteomics |
Contact List
Philippe Holzmuller |
contact affiliation | UMR 117 CIRAD-INRA ASTRE Montpellier, France |
contact email | philippe.holzmuller@cirad.fr |
lab head | |
Oana Vigy |
contact affiliation | FPP CNRS |
contact email | oana.vigy@fpp.cnrs.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007821
- Label: PRIDE project
- Name: Proteomics study of Amblyomma variegatum saliva, characterization of the molecular determinants.