PXD007821-1

PXD007821 is an original dataset announced via ProteomeXchange.

Title	Proteomics study of Amblyomma variegatum saliva, characterization of the molecular determinants.
Description	Amblyomma variegatum, the tropical bont tick, is a species of veterinary importance being considered as one of major pest of ruminants in Africa and in the Caribbean. It is associated with severe dermatophilosis and transmits the rickettsiale Ehrlichia ruminantium, the agent of heartwater, to ruminants. Tick’s saliva contains a complex mixture of bioactive molecules including proteins that modulate host responses to ensure successful blood feeding. The limited amount of saliva that can be obtained from ticks has hampered characterisation of salivary proteins using traditional protein chemistry, but recent advances in proteomics (especially mass spectrometry and bioinformatics) provide new tools to finely investigate tick sialomes. The general objective of this study was to better understand the role of A. variegatum saliva in tick/host/pathogen interactions. Salivation of semi-fed females was induced after detachment by injection of pilocarpin. Crude saliva proteins were pre-fractioned by SDS-PAGE and trypsin-generated peptides were analysed online by nano-flow Ultimate 3000 HPLC–nanoelectrospray ionization using an Orbitrap Elite mass spectrometer. Raw data analysis was performed using the MaxQuant software and proteins identified according to UniProt Knowledgebase. Our results bring new insights for a better understanding of tick-ruminant interactions at the molecular level, and pave the way towards integrative strategies to interfere with both the immunomosuppressive and infectious processes in corresponding tick-borne diseases.
HostingRepository	PRIDE
AnnounceDate	2018-01-05
AnnouncementXML	Submission_2018-01-05_01:42:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oana Vigy
SpeciesList	scientific name: Amblyomma; NCBI TaxID: 6942; scientific name: Ixodes scapularis (Black-legged tick) (Deer tick); NCBI TaxID: 6945;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-22 08:24:30	ID requested
⏵ 1	2018-01-05 01:42:28	announced
2	2018-03-07 23:57:12	announced	Updated publication reference for PubMed record(s): 29354598.
3	2024-10-22 04:40:56	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Amblyomma variegatum, tick saliva, immunomodulation, proteomics

Philippe Holzmuller
contact affiliation	UMR 117 CIRAD-INRA ASTRE Montpellier, France
contact email	philippe.holzmuller@cirad.fr
lab head
Oana Vigy
contact affiliation	FPP CNRS
contact email	oana.vigy@fpp.cnrs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007821
     1. Label: PRIDE project
     2. Name: Proteomics study of Amblyomma variegatum saliva, characterization of the molecular determinants.