PXD006381-2

PXD006381 is an original dataset announced via ProteomeXchange.

Title	Proteome of the Developing Murine Lens Through Mass Spectrometry
Description	The proteome is a term used for the entire catalog of proteins present in a cell at any time. In here, we investigated the proteome of developing mouse lens through mass spectrometry-based protein sequencing. We extracted mouse lenses at six developmental time points, which included two embryonic (E15 and E18) and four postnatal stages (P0, P3, P6, and P9). The lenses from each time point were preserved in three distinct pools to serve as biological replicates for each developmental stage. The total cellular protein was extracted from the lens, digested with trypsin and labeled with 6-plex isobaric tandem mass tags (TMT) for three independent 6-plex TMT experiments. A total of 6,117 proteins were identified in the mouse ocular lens in at least one of the above-mentioned six developmental time points. Of these, 6,117 proteins were present in one TMT set, 4,325 in two, and 2,972 were present in three TMT sets, respectively. Majority of the proteins exhibit steady expression; however, we identified 162 proteins that exhibited an 8-fold differential (higher or lower) expression during the developmental time course compared to their levels at E15. The lens proteome is comprised of diverse proteins that have distinct biological properties and functional characteristics including a total of 404 proteins that have been associated with autophagy. We have established a comprehensive profile of the developing murine lens proteome. This repository will be helpful in identifying critical components of lens development and processes essential for the maintenance of its transparency.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:36:58.722.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	OM Genetics
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-25 08:06:31	ID requested
1	2018-01-22 04:11:37	announced
⏵ 2	2024-10-22 04:36:59	announced	2024-10-22: Updated project metadata.

Khan SY, Ali M, Kabir F, Renuse S, Na CH, Talbot CC, Hackett SF, Riazuddin SA, Proteome Profiling of Developing Murine Lens Through Mass Spectrometry. Invest Ophthalmol Vis Sci, 59(1):100-107(2018) [pubmed]

10.1167/iovs.17-21601;

curator keyword: Biological

submitter keyword: Mouse ocular lens, Mass-spectrometry, Proteome

S. Amer Riazuddin
contact affiliation	The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287 USA
contact email	sriazud1@jhmi.edu
lab head
OM Genetics
contact affiliation	Johns Hopkins Wilmer Eye Institute
contact email	omgenetics@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006381
     1. Label: PRIDE project
     2. Name: Proteome of the Developing Murine Lens Through Mass Spectrometry