PXD006381 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome of the Developing Murine Lens Through Mass Spectrometry |
Description | The proteome is a term used for the entire catalog of proteins present in a cell at any time. In here, we investigated the proteome of developing mouse lens through mass spectrometry-based protein sequencing. We extracted mouse lenses at six developmental time points, which included two embryonic (E15 and E18) and four postnatal stages (P0, P3, P6, and P9). The lenses from each time point were preserved in three distinct pools to serve as biological replicates for each developmental stage. The total cellular protein was extracted from the lens, digested with trypsin and labeled with 6-plex isobaric tandem mass tags (TMT) for three independent 6-plex TMT experiments. A total of 6,117 proteins were identified in the mouse ocular lens in at least one of the above-mentioned six developmental time points. Of these, 6,117 proteins were present in one TMT set, 4,325 in two, and 2,972 were present in three TMT sets, respectively. Majority of the proteins exhibit steady expression; however, we identified 162 proteins that exhibited an 8-fold differential (higher or lower) expression during the developmental time course compared to their levels at E15. The lens proteome is comprised of diverse proteins that have distinct biological properties and functional characteristics including a total of 404 proteins that have been associated with autophagy. We have established a comprehensive profile of the developing murine lens proteome. This repository will be helpful in identifying critical components of lens development and processes essential for the maintenance of its transparency. |
HostingRepository | PRIDE |
AnnounceDate | 2018-01-22 |
AnnouncementXML | Submission_2018-01-22_04:11:35.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | OM Genetics |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-04-25 08:06:31 | ID requested | |
⏵ 1 | 2018-01-22 04:11:37 | announced | |
2 | 2024-10-22 04:36:59 | announced | 2024-10-22: Updated project metadata. |
Publication List
Khan SY, Ali M, Kabir F, Renuse S, Na CH, Talbot CC, Hackett SF, Riazuddin SA, Proteome Profiling of Developing Murine Lens Through Mass Spectrometry. Invest Ophthalmol Vis Sci, 59(1):100-107(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Mouse ocular lens, Proteome, Mass-spectrometry |
Contact List
S. Amer Riazuddin |
contact affiliation | The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287 USA |
contact email | sriazud1@jhmi.edu |
lab head | |
OM Genetics |
contact affiliation | Johns Hopkins Wilmer Eye Institute |
contact email | omgenetics@jhmi.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD006381 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006381
- Label: PRIDE project
- Name: Proteome of the Developing Murine Lens Through Mass Spectrometry