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PXD005163-5

PXD005163 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIn-depth membrane proteomic study of breast cancer tissues
DescriptionHuman breast cancer tissue samples from 18 patients with high-risk or low-risk MammaPrint breast cancer were analyzed. The membrane fractions were enriched, and the extracted proteins were reduced, alkylated, digested with Lys-C and Trypsin, and labelled with iTRAQ4plex: 114; high risk; 115; low risk. Identification results were filtered at 1% false discovery rate (FDR) thresholds by searching against a randomized decoy database using Mascot at peptide and protein levels.
HostingRepositoryjPOST
AnnounceDate2016-10-17
AnnouncementXMLSubmission_2018-09-18_23:41:04.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD005163
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMariko Ito
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; iTRAQ4plex-116 reporter+balance reagent N6-acylated lysine; iTRAQ4plex-116 reporter+balance reagent acylated N-terminal; iTRAQ4plex-116 reporter+balance reagent O4'-acylated tyrosine
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-10-17 01:22:12ID requested
12016-10-17 04:05:10announced
22016-11-17 18:13:15announcedUpdated description.
32017-03-30 23:34:44announcedUpdated Repository support.
42017-03-31 00:12:18announcedUpdated DigitalObjectIdentifier.
52018-09-18 23:41:05announcedUpdated CV, FTP location and Modification list.
62021-02-04 17:13:27announced2021-02-04: Updated FTP location.
72022-09-18 03:21:49announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: membrane protein
breast cancer tissues
shotgun proteomics
Contact List
Takeshi Tomonaga
lab head
Mariko Ito
contact affiliationKyoto Univ.
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://jpost.pharm.kyoto-u.ac.jp/repository/JPST000201