PXD005163-4

PXD005163 is an original dataset announced via ProteomeXchange.

Title	In-depth membrane proteomic study of breast cancer tissues
Description	Human breast cancer tissue samples from 18 patients with high-risk or low-risk MammaPrint breast cancer were analyzed. The membrane fractions were enriched, and the extracted proteins were reduced, alkylated, digested with Lys-C and Trypsin, and labelled with iTRAQ4plex: 114; high risk; 115; low risk. Identification results were filtered at 1% false discovery rate (FDR) thresholds by searching against a randomized decoy database using Mascot at peptide and protein levels.
HostingRepository	jPOST
AnnounceDate	2016-10-17
AnnouncementXML	Submission_2017-03-31_00:12:18.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005163
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mariko Ito
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; Applied Biosystems iTRAQ(TM) multiplexed quantitation chemistry; (2S,3R)-3-hydroxyaspartic acid; iTRAQ4plex
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-17 01:22:12	ID requested
1	2016-10-17 04:05:10	announced
2	2016-11-17 18:13:15	announced	Updated description.
3	2017-03-30 23:34:44	announced	Updated Repository support.
⏵ 4	2017-03-31 00:12:18	announced	Updated DigitalObjectIdentifier.
5	2018-09-18 23:41:05	announced	Updated CV, FTP location and Modification list.
6	2021-02-04 17:13:27	announced	2021-02-04: Updated FTP location.
7	2022-09-18 03:21:49	announced	2022-09-18: Updated FTP location.

Dataset with its publication pending

submitter keyword: membrane protein

breast cancer tissues

shotgun proteomics

Takeshi Tomonaga
lab head
Mariko Ito
contact affiliation	Kyoto Univ.
dataset submitter

jPOST dataset URI