PXD004621-3

PXD004621 is an original dataset announced via ProteomeXchange.

Title	Human Fibroblast cell_aHDF1419-P10
Description	Proteome analyses of human fibroblast cell line (aHDF1419-P10) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.
HostingRepository	jPOST
AnnounceDate	2016-07-21
AnnouncementXML	Submission_2017-03-30_23:31:23.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michio Funahashi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; (2S,3R)-3-hydroxyaspartic acid
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-20 19:48:56	ID requested
1	2016-07-20 20:48:19	announced
2	2016-11-17 18:10:32	announced	Updated description.
⏵ 3	2017-03-30 23:31:24	announced	Updated Repository support.
4	2017-03-31 00:04:47	announced	Updated DigitalObjectIdentifier.
5	2018-09-18 22:41:05	announced	Updated CV, FTP location and Modification list.
6	2021-02-04 17:22:31	announced	2021-02-04: Updated FTP location.
7	2022-09-18 02:41:59	announced	2022-09-18: Updated FTP location.

Dataset with its publication pending

submitter keyword: shotgun proteomics, monolithic silica column, iPS cell, one-shot proteomics

Yasushi Ishihama
lab head
Michio Funahashi
contact affiliation	Kyoto university
dataset submitter

jPOST dataset URI