PXD004621-2
PXD004621 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Human Fibroblast cell_aHDF1419-P10 |
| Description | Proteome analyses of human fibroblast cell line (aHDF1419-P10) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project. |
| HostingRepository | jPOST |
| AnnounceDate | 2016-07-21 |
| AnnouncementXML | Submission_2016-11-17_18:10:31.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Michio Funahashi |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | iodoacetamide derivatized residue; (2S,3R)-3-hydroxyaspartic acid |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2016-07-20 19:48:56 | ID requested | |
| 1 | 2016-07-20 20:48:19 | announced | |
| ⏵ 2 | 2016-11-17 18:10:32 | announced | Updated description. |
| 3 | 2017-03-30 23:31:24 | announced | Updated Repository support. |
| 4 | 2017-03-31 00:04:47 | announced | Updated DigitalObjectIdentifier. |
| 5 | 2018-09-18 22:41:05 | announced | Updated CV, FTP location and Modification list. |
| 6 | 2021-02-04 17:22:31 | announced | 2021-02-04: Updated FTP location. |
| 7 | 2022-09-18 02:41:59 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: shotgun proteomics, monolithic silica column, iPS cell, one-shot proteomics |
Contact List
| Yasushi Ishihama | |
|---|---|
| lab head | |
| Michio Funahashi | |
| contact affiliation | Kyoto university |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
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