PXD004620-5
PXD004620 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human Fibroblast cell_aHDF1388-P9 |
Description | Proteome analyses of human fibroblast cell line (aHDF1388-P9) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project. |
HostingRepository | jPOST |
AnnounceDate | 2016-07-21 |
AnnouncementXML | Submission_2018-09-18_22:39:59.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD004620 |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Michio Funahashi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2016-07-20 19:48:23 | ID requested | |
1 | 2016-07-20 20:48:11 | announced | |
2 | 2016-11-17 18:09:55 | announced | Updated description. |
3 | 2017-03-30 23:30:44 | announced | Updated Repository support. |
4 | 2017-03-31 00:03:39 | announced | Updated DigitalObjectIdentifier. |
⏵ 5 | 2018-09-18 22:39:59 | announced | Updated CV, FTP location and Modification list. |
6 | 2021-02-04 17:21:38 | announced | 2021-02-04: Updated FTP location. |
7 | 2022-09-18 03:47:20 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: shotgun proteomics, monolithic silica column, iPS cell, one-shot proteomics |
Contact List
Yasushi Ishihama | |
---|---|
lab head | |
Michio Funahashi | |
contact affiliation | Kyoto university |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://jpost.pharm.kyoto-u.ac.jp/repository/JPST000087 |