PXD004620-3
PXD004620 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human Fibroblast cell_aHDF1388-P9 |
Description | Proteome analyses of human fibroblast cell line (aHDF1388-P9) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project. |
HostingRepository | jPOST |
AnnounceDate | 2016-07-21 |
AnnouncementXML | Submission_2017-03-30_23:30:44.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Michio Funahashi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue; (2S,3R)-3-hydroxyaspartic acid |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2016-07-20 19:48:23 | ID requested | |
1 | 2016-07-20 20:48:11 | announced | |
2 | 2016-11-17 18:09:55 | announced | Updated description. |
⏵ 3 | 2017-03-30 23:30:44 | announced | Updated Repository support. |
4 | 2017-03-31 00:03:39 | announced | Updated DigitalObjectIdentifier. |
5 | 2018-09-18 22:39:59 | announced | Updated CV, FTP location and Modification list. |
6 | 2021-02-04 17:21:38 | announced | 2021-02-04: Updated FTP location. |
7 | 2022-09-18 03:47:20 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: shotgun proteomics, monolithic silica column, iPS cell, one-shot proteomics |
Contact List
Yasushi Ishihama | |
---|---|
lab head | |
Michio Funahashi | |
contact affiliation | Kyoto university |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |