PXD004362 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomics of liver from PCB 153 exposed Atlantic cod |
Description | In order to investigate the underlying mechanisms of PCB 153 mediated toxicity to Atlantic cod (Gadus morhua), we analyzed the liver proteome of fish exposed to various doses of PCB 153 (0, 0.5, 2 and 8mg/kg body weight) for two weeks and examined the effects on expression of liver proteins using quantitative proteomics. Label-free mass spectrometry enabled quantification of 1272 proteins, and 78 were differentially regulated between PCB 153 treated samples and controls. Two proteins downregulated due to PCB 153 treatment, Glutathione S-transferase theta 1 (GSTT1) and sulfotransferase family protein 1 (ST2B1), were verified using selected reaction monitoring (SRM). Supported by bioinformatics analyses, we concluded that PCB 153 perturbs lipid metabolism in the Atlantic cod liver and that increased levels of lipogenic enzymes indicate increased synthesis of fatty acids and triglycerides. |
HostingRepository | PRIDE |
AnnounceDate | 2017-01-17 |
AnnouncementXML | Submission_2017-06-12_07:59:35.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Odd Andre Karlsen |
SpeciesList | scientific name: Gadus morhua (Atlantic cod); NCBI TaxID: 8049; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos; QTRAP 5500 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-06-20 01:27:21 | ID requested | |
1 | 2017-01-17 05:48:00 | announced | |
⏵ 2 | 2017-06-12 07:59:37 | announced | Updated publication reference for PubMed record(s): 28183064. |
Publication List
Yadetie F, Oveland E, D, ø, skeland A, Berven F, Goks, ø, yr A, Karlsen OA, Quantitative proteomics analysis reveals perturbation of lipid metabolic pathways in the liver of Atlantic cod (Gadus morhua) treated with PCB 153. Aquat Toxicol, 185():19-28(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Atlantic cod (Gadus morhua), PCB 153, Liver, Label-free, Orbitrap Velos Pro, SRM, 5500 QTRAP |
Contact List
Odd Andre Karlsen |
contact affiliation | Department of Biology, University of Bergen, Bergen, Norway |
contact email | Odd.Karlsen@uib.no |
lab head | |
Odd Andre Karlsen |
contact affiliation | Department of Biology, University of Bergen, Norway |
contact email | odd.karlsen@uib.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004362
- Label: PRIDE project
- Name: Quantitative proteomics of liver from PCB 153 exposed Atlantic cod