PXD004362-1

PXD004362 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of liver from PCB 153 exposed Atlantic cod
Description	In order to investigate the underlying mechanisms of PCB 153 mediated toxicity to Atlantic cod (Gadus morhua), we analyzed the liver proteome of fish exposed to various doses of PCB 153 (0, 0.5, 2 and 8mg/kg body weight) for two weeks and examined the effects on expression of liver proteins using quantitative proteomics. Label-free mass spectrometry enabled quantification of 1272 proteins, and 78 were differentially regulated between PCB 153 treated samples and controls. Two proteins downregulated due to PCB 153 treatment, Glutathione S-transferase theta 1 (GSTT1) and sulfotransferase family protein 1 (ST2B1), were verified using selected reaction monitoring (SRM). Supported by bioinformatics analyses, we concluded that PCB 153 perturbs lipid metabolism in the Atlantic cod liver and that increased levels of lipogenic enzymes indicate increased synthesis of fatty acids and triglycerides.
HostingRepository	PRIDE
AnnounceDate	2017-01-17
AnnouncementXML	Submission_2017-01-17_05:48:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Odd Andre Karlsen
SpeciesList	scientific name: Gadus morhua (Atlantic cod); NCBI TaxID: 8049;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; QTRAP 5500

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-20 01:27:21	ID requested
⏵ 1	2017-01-17 05:48:00	announced
2	2017-06-12 07:59:37	announced	Updated publication reference for PubMed record(s): 28183064.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Atlantic cod (Gadus morhua), PCB 153, Liver, Label-free, Orbitrap Velos Pro, SRM, 5500 QTRAP

Odd Andre Karlsen
contact affiliation	Department of Biology, University of Bergen, Bergen, Norway
contact email	Odd.Karlsen@uib.no
lab head
Odd Andre Karlsen
contact affiliation	Department of Biology, University of Bergen, Norway
contact email	odd.karlsen@uib.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD004362

PRIDE project URI

• PRIDE
  1. PXD004362
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of liver from PCB 153 exposed Atlantic cod