PXD004356-2

PXD004356 is an original dataset announced via ProteomeXchange.

Title	Defining the interactome of human adenine nucleotide translocases
Description	Adenine nucleotide translocases (ANT in mammals, AAC in yeast) mediate the 1:1 electrogenic exchange of ADP into and ATP out of the mitochondrial matrix and is thus a required component of the oxidative phosphorylation machinery. Originally modeled to function in isolation, the major isoform in Saccharomyces cerevisiae, AAC2, was recently demonstrated to associate with a number of protein complexes, including other mitochondrial solute carriers, higher order respiratory complexes (termed supercomplexes), and itself. As very little is known about the mammalian ANT interactome, we sought to define it using a proteomics-based approach. Here, we show that human ANT1 and ANT2, like yeast AAC2, do not function in isolation but have numerous binding partners including the respiratory supercomplex, Despite the differences in the organization of respiratory supercomplexes in yeast and mammals, wherein the latter additionally involves Complex I, the capacity of both AACs and ANTs to associate with it suggests an evolutionarily conserved interaction that has a functional importance.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:34:46.695.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Claypool
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-17 06:54:38	ID requested
1	2022-03-01 10:01:16	announced
⏵ 2	2024-10-22 05:34:47	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: ANT2, ANT1 Interactome, Proteomics

Steven M Claypool
contact affiliation	Steven M Claypool, Department of Physiology, Johns HopkinsUniversity School of Medicine, Baltimore, Maryland 21205 USA
contact email	sclaypo1@jhmi.edu
lab head
Steven Claypool
contact affiliation	Johns Hopkins University School of Medicine
contact email	sclaypo1@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004356
     1. Label: PRIDE project
     2. Name: Defining the interactome of human adenine nucleotide translocases