PXD004356 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Defining the interactome of human adenine nucleotide translocases |
Description | Adenine nucleotide translocases (ANT in mammals, AAC in yeast) mediate the 1:1 electrogenic exchange of ADP into and ATP out of the mitochondrial matrix and is thus a required component of the oxidative phosphorylation machinery. Originally modeled to function in isolation, the major isoform in Saccharomyces cerevisiae, AAC2, was recently demonstrated to associate with a number of protein complexes, including other mitochondrial solute carriers, higher order respiratory complexes (termed supercomplexes), and itself. As very little is known about the mammalian ANT interactome, we sought to define it using a proteomics-based approach. Here, we show that human ANT1 and ANT2, like yeast AAC2, do not function in isolation but have numerous binding partners including the respiratory supercomplex, Despite the differences in the organization of respiratory supercomplexes in yeast and mammals, wherein the latter additionally involves Complex I, the capacity of both AACs and ANTs to associate with it suggests an evolutionarily conserved interaction that has a functional importance. |
HostingRepository | PRIDE |
AnnounceDate | 2022-03-01 |
AnnouncementXML | Submission_2022-03-01_10:01:16.257.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Steven Claypool |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | 6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-06-17 06:54:38 | ID requested | |
⏵ 1 | 2022-03-01 10:01:16 | announced | |
2 | 2024-10-22 05:34:47 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: ANT2, ANT1 Interactome, Proteomics |
Contact List
Steven M Claypool |
contact affiliation | Steven M Claypool, Department of Physiology, Johns HopkinsUniversity School of Medicine, Baltimore, Maryland 21205 USA |
contact email | sclaypo1@jhmi.edu |
lab head | |
Steven Claypool |
contact affiliation | Johns Hopkins University School of Medicine |
contact email | sclaypo1@jhmi.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004356
- Label: PRIDE project
- Name: Defining the interactome of human adenine nucleotide translocases