PXD003865-2

PXD003865 is an original dataset announced via ProteomeXchange.

Title	mouse stem cell SWATH-MS .
Description	Adult stem cells (SCs) are essential for tissue maintenance and regeneration yet are susceptible to SC senescence during aging. Here we demonstrate the importance of cellular NAD+ level and its impact on mitochondrial activity as a pivotal switch to modulate muscle stem cell (MuSC) senescence. Importantly, the induction of the mitochondrial unfolded protein response (UPRmt) and of prohibitin proteins, subsequent to increasing cellular NAD+ with the precursor nicotinamide riboside (NR), rejuvenates MuSCs in aged mice. NR also prevents MuSCs senescence in Mdx mice, a mouse model of muscular dystrophy. Extending these observations to other SC pools and on the organism as a whole, we demonstrate that NR delays neural stem cell (NSC) and melanocyte stem cell (McSC) senescence, while also increasing mouse lifespan. Strategies that conserve cellular NAD+ could therefore be utilized to reprogram dysfunctional SCs in aging and disease to improve lifespan in mammals
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:33:10.573.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yibo Wu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-29 04:31:22	ID requested
1	2024-10-07 10:04:16	announced
⏵ 2	2024-10-22 05:33:11	announced	2024-10-22: Updated project metadata.

Zhang H, Ryu D, Wu Y, Gariani K, Wang X, Luan P, D'Amico D, Ropelle ER, Lutolf MP, Aebersold R, Schoonjans K, Menzies KJ, Auwerx J, repletion improves mitochondrial and stem cell function and enhances life span in mice. Science, 352(6292):1436-43(2016) [pubmed]

10.1126/science.aaf2693;

curator keyword: Biological

submitter keyword: mouse stem cell, SWATH-MS

Ruedi Aebersold
contact affiliation	IMSB, ETH Zurich, Switzerland
contact email	aebersold@imsb.biol.ethz.ch
lab head
Yibo Wu
contact affiliation	Biology
contact email	wu@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD003865

PRIDE project URI

• PRIDE
  1. PXD003865
     1. Label: PRIDE project
     2. Name: mouse stem cell SWATH-MS